激光生物学报2025，Vol.34Issue(1)：63-76,14.DOI:10.3969/j.issn.1007-7146.2025.01.008
肠上皮化生中铜死亡相关基因的鉴定和免疫学特征
Identification and Immunological Characterization of Genes Associated with Cuproptosis in Intestinal Metaplasia
摘要
Abstract
This study examines the association between intestinal metaplasia(IM)and cuproptosis,and develops a predictive model.By analyzing the GSE2669 dataset,gene clusters and immune characteristics associated with cuproptosis in 22 IM samples were characterized,with differential gene clusters identified.The performance of four machine learning models:random forest(RF),support vector machine(SVM),generalized linear model(GLM),and XGBoost(XGB),was evaluated with the RF model showing superior performance.A RF model based on four genes(DMBT1,HPN,SLC13A3,and YWHAZ)was constructed,and their expression was found to be significantly correlated with CDX2 levels.Expression levels of these genes in normal gastric and intestinal metaplastic tissues were confirmed by qRT-PCR and Western blot,aligning with the model's predictions.This research preliminarily identifies the role and potential mechanisms of cuproptosis in IM and establishes a predictive model,offering new directions for future clinical applications and research.关键词
铜死亡/肠上皮化生/机器学习模型/免疫浸润/预测模型Key words
cuproptosis/intestinal metaplasia/machine learning models/immune infiltration/prediction model分类
临床医学引用本文复制引用
钱菲菲,陈松,张里,钟洁,费素娟,庞训雷..肠上皮化生中铜死亡相关基因的鉴定和免疫学特征[J].激光生物学报,2025,34(1):63-76,14.基金项目
江苏省研究生科研与实践创新计划项目(SJCX23_1385) （SJCX23_1385）
徐州市科技局重点研发计划项目(KC22233) （KC22233）
江苏省高校重点实验室开放课题项目(XZSYSKF2021029) （XZSYSKF2021029）
徐州医科大学附属医院课题项目(2020KA003). （2020KA003）
