激光生物学报2025，Vol.34Issue(1)：84-89,6.DOI:10.3969/j.issn.1007-7146.2025.01.010
银杏内酯B介导IKK/NF-κB通路对肺腺癌A549细胞增殖及凋亡的调控作用
Ginkgolide B Regulates the Proliferation and Apoptosis of Lung Adenocarcinoma A549 Cells Through IKK/NF-κB Pathway
摘要
Abstract
To investigate the effect of ginkgolide B on the proliferation and apoptosis of human lung adenocarcinoma(A549)cells and the regulatory effect of IκB kinase/nuclear factor κB(IKK/NF-κB)signaling pathway.A549 cells were cultured in vitro and divided into control group(same volume DMSO solvent)and different concentrations(4,8,16,32 μmol/L)of ginkgolide B group.After 24 hours of intervention,cell counting kit-8(CCK-8)was used to detect cell viability to screen the optimal concentration of ginkgolide B for subsequent experiments.Then A549 cells were divided into control group,ginkgolide B group(16 μmol/L ginkgolide B),positive drug group(0.4 mg/L adriamycin),inhibitor group(16 μmol/L ginkgolide B+10 μmol/L IKK/NF-κB pathway inhibitor BAY11-7082)and activator group(16 μmol/L ginkgolide B+1 μmol/L IKK/NF-κB pathway activator Prostratin),intervention for 24 hours.5-ethynyl-2′-deoxyuridine(EdU)was used to measure cell proliferation.Hoechst 33258 staining kit was used to detect cell apoptosis.Western blot(WB)was used to detect the expression levels of CyclinD1,Caspase-3 and IKK/NF-κB pathway-related proteins.According to the results of CCK-8 experiment,16 μmol/L ginkgolide B was selected for subsequent experiments.Compared with the control group,the proliferation rate of A549 cells and the protein expression levels of CyclinD1,p-NF-κB p65/NF-κB p65 and p-IκBα/IκBα in the ginkgolide B group and the positive drug group significantly decreased(P<0.05),and the apoptosis rate and Caspase-3 protein expression level significantly increased(P<0.05).Compared with ginkgolide B group,cell proliferation rate,CyclinD1,p-NF-κB p65/NF-κB p65 and p-IκBα/IκBα ratio significantly decreased in inhibitor group(P<0.05),while apoptosis rate and Caspase-3 protein expression level significantly increased(P<0.05).In the activator group,cell proliferation rate,CyclinD1,p-NF-κB p65/NF-κB p65 and p-IκBα/IκBα ratios significantly increased(P<0.05),while apoptosis rate and Caspase-3 protein expression levels significantly decreased(P<0.05).Ginkgolide B can inhibit the proliferation and induce apoptosis of A549 cells by regulating IKK/NF-κB pathway signaling.关键词
肺腺癌/银杏内酯B/IκB激酶/核因子κB信号通路/细胞增殖/细胞凋亡Key words
adenocarcinoma of the lung/ginkgolide B/IκB kinase/nuclear factor-κB signaling pathway/cell proliferation/cell apoptosis分类
临床医学引用本文复制引用
徐林艳,曾怡兰,喻雅真..银杏内酯B介导IKK/NF-κB通路对肺腺癌A549细胞增殖及凋亡的调控作用[J].激光生物学报,2025,34(1):84-89,6.基金项目
湖南省残联康复课题项目(2023XK0312). （2023XK0312）
