摘要
Abstract
Objective To explore the analgesic mechanism of Shougan San based on network pharmacology research meth-ods.Methods The TCMSP database was used to search for effective components and potential targets of Shaogan San,the GeneCards database was used to collect targets of pain diseases and obtain intersection targets with drugs.Cytoscape 3.9.1 was used to construct a drug-component-intersection target network diagram,selecting core components.String database was used to draw protein interaction network diagrams and select core targets.David database was used for GO functional enrichment analysis and KEGG pathway enrichment analysis.Results There are 167 effective components in Shaogan San,with 224 intersection between potential targets and pain targets.The"drug-component-target-disease"network suggests that the core compounds of Shaogan San are quercetin,kaempferol,7-methoxy-2-methyl isoflavones,β-sitosterol,narin-genin,isorhamneti,formononetin,Medicarpin,licochalcone A,stigmasterol.The"drug-component-target-disease"network combined with the protein interaction network indicates that estrogen receptor,COX2,heat shock protein 90,calmodulin,protein kinase,interleukin-6,cancer suppressor protein,interleukin-1 β may be key targets.KEGG pathway enrichment analysis showed that the core pathways mainly included cancer signaling pathway,lipid and atherosclerosis signaling pathway,AGE-RAGE signaling pathway in diabetes complications,fluid shear stress and atherosclerosis signaling pathway.Conclusion Shaogan San may play an analgesic role through the interaction of multi-target and multi-signal pathways,which can provide a basis for further experimental research.关键词
网络药理学/芍甘散/镇痛/经验方Key words
Network pharmacology/Shaogan San/Analgesia/Experienced prescription分类
中医学
