中国当代医药2025，Vol.32Issue(6)：4-9,6.DOI:10.3969/j.issn.1674-4721.2025.06.01
基于超高效液相色谱-四极杆飞行时间质谱联用技术分析芍丹颗粒中化学成分
Analysis of chemical composition in Shaodan Granules by UHPLC-Q-TOF-MS
林松 1林小青2
作者信息
- 1. 福州市第二总医院妇幼保健院药剂科,福建 福州 350008
- 2. 福建中医药大学药学院,福建 福州 350003
- 折叠
摘要
Abstract
Objective The chemical composition of Shaodan Granules are analyzed by ultra-high performance liquid chro-matography-quadrupole time-of-flight mass spectrometry(hereinafter referred to as"UHPLC-Q-TOF-MS").Methods The solution of Shaodan Granules was ionized by electrospray ionization(ESI),positive and negative ion pattern scanning,and the molecular ion peak and secondary mass spectrometry fragment ion peak of the chemical composition of Shaodan Granules were determined.The source and attribution of the chemical composition of Shaodan Granules were analyzed by comparing the mass spectrometry fragment ion peak and retention time of the sample with 15 reference products and literature data.Results The measured value of molecular ion peak,ion form,molecular formula and secondary mass spec-trometry characteristic fragments obtained by mass spectrometry were compared with PubMed,CNGI,SeiFinder and other databases and reference materials.A total of 5 lactones,4 phenolic acids,2 phenanthrene quinone,3 alkaloids and terpenes such as monoterpenes,sesquiterpenes and diterpenes were identified from Shaodan Granules.A total of 33 components,such as organic acids were determined to be derived from Shaodan Granules.Conclusion The established UH-PLC-Q-TOF-MS qualitative analysis method can detect the chemical composition of Shaodan Granules easily and quickly,and provide a basis for the quality control and follow-up research and development of Shaodan Granules.关键词
芍丹颗粒/主成分分析/药物研发/药品评价Key words
Shaodan Granules/Principal component analysis/Drug development/Drug evaluation分类
医药卫生引用本文复制引用
林松,林小青..基于超高效液相色谱-四极杆飞行时间质谱联用技术分析芍丹颗粒中化学成分[J].中国当代医药,2025,32(6):4-9,6.
