扬州大学学报（农业与生命科学版）2025，Vol.46Issue(1)：37-43,7.DOI:10.16872/j.cnki.1671-4652.2025.01.005
表达传染性喉气管炎病毒gB蛋白重组耐热新城疫病毒的构建
Generation of the thermostable recombinant Newcastle disease virus expressing the gB protein of infectious laryngotracheitis virus
摘要
Abstract
To generate a thermostable and combined vaccine candidate against the infectious laryngotracheitis(ILT)and Newcastle disease(ND),this study cloned the gB gene of ILTV to the thermostable NDV NDV/rAHR09 strain using the seamless cloning technique.Then,the Western blot experiment was used to check the expression of gB,and the thermostable and virulence assessment experiments were used to detect the biological characteristics of the recombinant vi-rus.The results showed that the gB gene of ILTV was successfully inserted into the pNDV/rAHR09,and the rescued recombinant virus showed a high haemagglutination(HA)titer.Additionally,the Western blot assay confirmed the ex-pression of the gB protein.The thermostable assessment showed that the recombinant viruses maintained a high HA ac-tivity and virus titer after exposure to 56 ℃ for about 60 minutes.Pathogenicity assessment data indicated that the recom-binant virus had a mean death time(MDT)in chicken embryos of 148 hours,an intracerebral pathogenicity index(ICPI)of 0,and an intravenous pathogenicity index(IVPI)of 0.1,suggesting that the recombinant virus possessed typical char-acteristics of an attenuated NDV.In conclusion,the thermostable vaccine candidate for ILT and ND was successfully de-veloped in this study,which provides a novel approach for developing a live vector vaccine against ILTV.关键词
传染性喉气管炎病毒/gB基因/新城疫病毒/耐热/重组病毒Key words
infectious laryngotracheitis virus/gB gene/Newcastle disease virus/thermostable/recombinant virus分类
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薄宗义,胡海,韩成昊,蒋皓静,孟晨晨,张小荣,吴艳涛,曹永忠..表达传染性喉气管炎病毒gB蛋白重组耐热新城疫病毒的构建[J].扬州大学学报（农业与生命科学版）,2025,46(1):37-43,7.基金项目
江苏省农业科技自主创新资金项目[CX(21)3129] （21）
国家现代农业产业技术体系建设专项(CARS-40-K16) （CARS-40-K16）
