扬州大学学报（农业与生命科学版）2025，Vol.46Issue(1)：44-51,8.DOI:10.16872/j.cnki.1671-4652.2025.01.006
GETV E1蛋白单克隆抗体制备及抗原表位鉴定
Preparation and identification of monoclonal antibody against E1 protein of GETV
摘要
Abstract
In order to prepare a monoclonal antibody against the E1 protein of Getah virus(GETV),the hydrophilic region fragment(amino acid 31-182 region)of GETV E1 gene was constructed into pCold-TF prokaryotic expression vector in this study.After expression and purification,TF-E1 recombinant protein was prepared,and mice were immu-nized with it as an immunogen.With the help of hybridoma technology,one monoclonal antibody against GETV E1 pro-tein was successfully obtained.The biological characterization of the obtained E1 monoclonal antibody showed that the monoclonal antibody against E1 protein prepared in this study was suitable for indirect immunofluorescence and Western blot characterization.Meanwhile,by truncating the expression of E1 protein,the antigenic epitope recognized by the E1 protein monoclonal antibody was further clarified to be 73RPDYQC78,and the comparison of this antigenic epitope with the sequence of GETV strains that had been uploaded in GenBank revealed that this sequence was highly conserved among GETV strains.In conclusion,the successful preparation of monoclonal antibody to GETV E1 protein provides an immu-nological tool for the study of GETV.关键词
盖塔病毒/E1蛋白/单克隆抗体/抗原表位Key words
Getah virus/E1 protein/monoclonal antibody/antigenic epitope分类
畜牧业引用本文复制引用
黄艳梅,孟慧,朱立麒,牟春晓,陈振海..GETV E1蛋白单克隆抗体制备及抗原表位鉴定[J].扬州大学学报（农业与生命科学版）,2025,46(1):44-51,8.基金项目
江苏省高等学校自然科学基金面上项目(20KJB230007) （20KJB230007）
江苏省研究生科研与实践创新计划项目(SJCX24_2306) （SJCX24_2306）
江苏高校优势学科建设工程项目(PAPD) （PAPD）
