扬州大学学报（农业与生命科学版）2025，Vol.46Issue(1)：70-78,9.DOI:10.16872/j.cnki.1671-4652.2025.01.009
多光谱及分子对接技术研究氯霉素与胰蛋白酶相互作用
Study on the interaction between chloramphenicol and trypsin by multispectral and molecular docking techniques
摘要
Abstract
In order to explore the interaction mechanism between chloramphenicol and trypsin,and to study the influ-ence of chloramphenicol on the structure and activity of trypsin,this paper studied the interaction between chlorampheni-col and trypsin under simulated physiological conditions by using multispectral and molecular docking.The results showed that the fluorescence intensity of trypsin decreased with the increase of chloramphenicol concentration.The fluorescence dynamic quenching constants(Ksv)decreased with the increase of temperature,indicating that chloramphenicol could effectively quenching the fluorescence of trypsin,and chloramphenicol quenched the fluorescence of trypsin through a stat-ic process.The thermodynamic parameters showed that the interaction force between chloramphenicol and trypsin was dominated by the electrostatic attraction model.Circular dichroism(CD)and Fourier transform infrared(FTIR)spectros-copy showed that chloramphenicol changed the secondary structure of trypsin.Enzyme activity studies showed that chlor-amphenicol caused a decrease in trypsin activity in the range of studied concentrations.Molecular docking showed that chloramphenicol can form hydrogen bonds with trypsin HIS-57,TYR-59 and SER-195,respectively.The results showed further evidence that the presence of chloramphenicol affects the structure of trypsin.In summary,chloramphenicol can interact with trypsin and affect the structure and activity of trypsin.This finding can serve as a solid foundation for future research into the in vivo interactions between antibiotic medications and digestive enzymes.关键词
多光谱技术/分子对接/胰蛋白酶/氯霉素/相互作用Key words
multispectral technique/molecular docking technique/trypsin/chloramphenicol/interaction分类
化学引用本文复制引用
郑英,郭倩,王冰,朱素娟..多光谱及分子对接技术研究氯霉素与胰蛋白酶相互作用[J].扬州大学学报（农业与生命科学版）,2025,46(1):70-78,9.基金项目
江苏省人兽共患病学重点实验室开放课题(R2209) （R2209）
江苏省研究生实践创新计划项目(SJCX21_1595) （SJCX21_1595）
