扬州大学学报（农业与生命科学版）2025，Vol.46Issue(1)：79-89,131,12.DOI:10.16872/j.cnki.1671-4652.2025.01.010
1株耐碳青霉烯类鲍曼不动杆菌BM5的生物学特征及基因组序列分析
Molecular characterization and comparative genomic analysis of a carbapenem-resistant Acinetobacter baumannii BM5
摘要
Abstract
In order to explore the genome and molecular biological characteristics of Acinetobacter baumannii,a clini-cally isolated pathogen was selected as the target strain and named BM5.The drug resistance of Acinetobacter baumannii was analyzed by drug sensitivity test,its genome was analyzed by high-throughput sequencing,its multi-site sequence typing(MLST),drug resistance genes and virulence genes were analyzed and predicted by relevant databases and bioin-formatics methods,and its evolutionary correlation with other Acinetobacter baumannii strains was determined by analy-zing and comparing genomic sequence similarity.The results showed that the whole genome was 3 990 385 bp circular DNA with GC content of 38.9％.The strain also contained two ring plasmids(72 258 and 8 731 bp in length).The MLST type of the bacterium was ST2,carring 85 drug resistance genes and including carbapenem resistance genes(96.63％),83 viru-lence genes and 5 prophages.Therefore,the bacterium may acquire and maintain its multi-drug resistance and complex pathogenicity through multiple pathways.In conclusion,the genomic characteristics of the new carbapenem-resistant AcinetobacterbaumanniiBM5 were analyzed,which will lay a foundation for further research on the mechanism of drug resistance and pathogenic mechanism of carbapenem-resistant Acinetobacter baumannii.关键词
耐碳青霉烯类/鲍曼不动杆菌/全基因组测序/耐药基因/毒力基因Key words
carbapenem resistant/Acinetobacter baumannii/whole genome sequencing/drug resistance gene/virulence gene分类
基础医学引用本文复制引用
张莹,支娅茹,于鑫焱,刘晓秋..1株耐碳青霉烯类鲍曼不动杆菌BM5的生物学特征及基因组序列分析[J].扬州大学学报（农业与生命科学版）,2025,46(1):79-89,131,12.基金项目
国家自然科学基金资助项目(81501797、31800156) （81501797、31800156）
