扬州大学学报（农业与生命科学版）2025，Vol.46Issue(1)：121-131,11.DOI:10.16872/j.cnki.1671-4652.2025.01.014
超声处理对泡核桃雄花总多酚提取效果的影响及其特性
Effects of ultrasound treatment on the extraction of Juglans sigillata Dode male flower total polyphenols and its characteristics
摘要
Abstract
The Juglans sigillata Dode male flower extracts can reduce the oxidative stress and inhibit the oxidation of macromolecules.In this study,on the basis of optimizing the better extraction conditions of male flower total polyphe-nols,its components and antioxidant effect in vitro were investigated,and the ultrasound mechanism was preliminarily described.Total polyphenols extraction conditions of male flowers was optimized by multiple quadratic regression equa-tion;its components were analyzed by HPLC;The antioxidant activity of total polyphenol was evaluated by determining the clearance of superoxide anion and hydroxyl radical;The microstructure of walnut male flowers powders before and af-ter ultrasound treatment was observed by SEM.The results showed that the ultrasound power was determined at 250 W,the better extraction process of total polyphenol of walnut male flowers was as follows:solid to liquid ratio 1:60,treat-ment time 21 min,ethanol concentration 50％,treatment temperature 61 ℃,the total polyphenol yield of walnut male flowers was(24.45±2.12)mg·g-1;Four kinds of monomeric phenol were identified by HPLC from walnut male flow-ers.Among them,chlorogenic acid and rutinum were higher,and gallic acid and quercetin were lower;total polyphenol,vitamin C(Vc)and BHT could remove superoxide anions and hydroxyl radicals.In terms of its clearance capability,Vc＞total polyphenol＞BHT;ultrasound treatment could tear and destroy the tissue surface of male flower,and release more plant polyphenol and strengthen the extraction effect.The results can provide the possibility for its application of male flowers polyphenols in the field of food storage and preservation.关键词
泡核桃/雄花总多酚/超声处理/抗氧化性Key words
Juglans sigillata Dode/male flowers total polyphenol/ultrasound treatment/antioxidation分类
农业科技引用本文复制引用
王纪辉,尹爽,耿阳阳,胡伯凯,曾亚军,卢中科..超声处理对泡核桃雄花总多酚提取效果的影响及其特性[J].扬州大学学报（农业与生命科学版）,2025,46(1):121-131,11.基金项目
贵州省科技计划项目[黔科合支撑(2022)重点014号] （2022）
贵州省林业科研项目[黔林科合J字(2024)11号、黔林科合J字(2022)04号] （2024）
贵州省木本粮油加工科技创新人才团队项目[黔科合平台人才(2020)5003] （2020）
贵州省核桃工程技术研究中心科研项目[黔科合平台人才(2019)5202号] （2019）
