现代畜牧兽医Issue(1)：1-5,5.DOI:10.20154/j.cnki.issn1672-9692.2025.01.001
羊源海藻糖比伯斯坦杆菌辽宁株的分离鉴定及其耐药性分析
Isolation,identification,and drug resistance analysis of Bibersteinia trehalosi-derived from sheep in Liaoning
摘要
Abstract
The aim of this study was to identify the pathogenicity and drug resistance of Bibersteinia trehalosi of sheep.The lung,liver and spleen disease samples of suspected infected lambs were collected aseptically,identified by bacteriological and molecular biological methods,and pathogenicity and drug susceptibility tests were conducted.The results showed that a strain of Bibersteinia trehalosi was isolated.The bacterium formed a round translucent colony with smooth convex surface and needle tip size on 10%sheep blood AGAR medium,and the two ends of the blunt round,single or paired Gram-negative small brachyrod-shaped bacteria could be observed under the microscope.The pathogenicity test results showed that the incidence rate and mortality rate of infected mice were 100%and 40%,showing pulmonary congestion and edema,epicardial congestion and hemorrhage,liver,spleen and kidney parenchymal organs congestion and degeneration,and intestinal mucosal flushing and swelling,which proved that the isolate had strong pathogenicity.Resistance analysis showed that the isolates were sensitive to 14 antibiotics,such as ampicillin and gentamicin,and resistant to ceftiofur and colistin.The results showed that the Liaoning strain of sheep-derived trehalose was highly pathogenic and sensitive to most antimicrobial agents.The results could provide reference for the diagnosis and drug resistance of sheep-derived trehalose.关键词
羊/海藻糖比伯斯坦杆菌/分离鉴定/致病性/耐药性/辽宁株Key words
Sheep/Bibersteinia trehalosi/Isolation and identification/Pathogenicity/Drug resistance/Liaoning strain分类
农业科技引用本文复制引用
李佳美,王辉暖,张利勃,刘海月,孟凡奇,吴靖,孙弋雅,周铁忠..羊源海藻糖比伯斯坦杆菌辽宁株的分离鉴定及其耐药性分析[J].现代畜牧兽医,2025,(1):1-5,5.基金项目
辽宁省建昌县牛羊科技特派团(2024JH5/10400039) （2024JH5/10400039）
辽宁省锦州高新区蛋鸡科技特派团(2024JH5/10400046) （2024JH5/10400046）
