现代畜牧兽医Issue(1)：6-9,4.DOI:10.20154/j.cnki.issn1672-9692.2025.01.002
不同繁殖能力藏羊卵巢COLIA1表达量的定量研究
Quantitative study of COLIA1 expression in ovarian of Tibetan sheep with different reproductive capacities
摘要
Abstract
The purpose of this study was to investigate the differences in the expression of type Ⅰ collagen gene α1(COLIA1)in the ovaries of Tibetan sheep with different reproductive ability,and to explore the correlation between the expression of COLIA1 and the reproductive ability of Tibetan sheep,so as to provide reference for improving the reproductive efficiency of Tibetan sheep.Twenty healthy Tibetan ewes aged 3 to 4 years with body weight of(54.45±1.25)kg were selected and divided into single and double lamb groups according to different lamb numbers(10 sheep per group),and ovarian samples were collected.Real-time quantitative fluorescent PCR(qRT-PCR)was used to detect the expression of COLIA1 gene in ovarian tissues of single and double lambs.The results showed that the expression level of COLIA1 gene in the ovaries of twin lambs was extremely higher than that of single lambs(P<0.01),suggesting that COLIA1 gene was closely related to the fertility of Tibetan sheep.Studies have shown that high expression of COLIA1 gene may promote follicular development and ovulation,thereby increasing the number of lambs in Tibetan sheep.关键词
藏羊/COLIA1基因/实时荧光定量PCR/产羔数Key words
Tibetan sheep/COLIA1 gene/Real-time fluorescence quantitative PCR/Lambing number分类
农业科技引用本文复制引用
金雲鑫,孙武,马世科,金夏阳,马玉红..不同繁殖能力藏羊卵巢COLIA1表达量的定量研究[J].现代畜牧兽医,2025,(1):6-9,4.基金项目
国家现代农业产业技术体系资助(CARS-39-35) （CARS-39-35）
省部共建三江源生态和高原农牧业国家重点实验室自主研究项目(2023-ZZ-11) （2023-ZZ-11）
青海省科技成果转化专项(2023-NK-132) （2023-NK-132）
青海省中青年科技人才托举工程(2023QHSKXRCTJ17) （2023QHSKXRCTJ17）
