现代畜牧兽医Issue(2)：1-5,5.DOI:10.20154/j.cnki.issn1672-9692.2025.02.001
狐源肺炎克雷伯菌辽宁分离株的鉴定及其致病性与耐药性分析
Identification,pathogenicity,and drug resistance analysis of the Liaoning isolate of fox-derived Klebsiella pneumoniae
摘要
Abstract
The study aimed to confirm the pathogenicity and drug resistance of fox-derived Klebsiella pneumoniae.Liver samples from diseased foxes at a fur farm in Jinzhou,Liaoning Province,were collected for bacteriological and molecular biological identification,pathogenicity testing,and antimicrobial susceptibility testing.The results showed that a strain of Klebsiella pneumoniae of fox origin(JMAV22.202201)was successfully isolated through bacteriological and molecular identification.On LB agar medium,the strain formed round,smooth,milky-white colonies.On MacConkey agar,pale pink,creamy colonies.And on sheep blood agar,round,smooth,gray-white colonies.Gram staining revealed pink-colored,short rod-shaped bacteria.Pathogenicity tests demonstrated 100％morbidity and mortality in infected mice,with multi-organ lesions including hemorrhaging in the lungs and spleen,swelling and congestive degeneration in the liver and kidneys,and congestive edema in the intestinal wall.Antimicrobial susceptibility testing indicated resistance to ampicillin,oxacillin,gentamicin,erythromycin,and trimethoprim-sulfamethoxazole,while susceptibility was observed to ceftriaxone,cefuroxime,tetracycline,minocycline,ciprofloxacin,levofloxacin,and florfenicol.The study concludes that the isolated strain exhibits strong pathogenicity but remains susceptible to most tested antimicrobial agents.关键词
狐/肺炎克雷伯菌/细菌分离鉴定/致病性/耐药性Key words
Fox/Klebsiella pneumoniae/Bacterial isolation and identification/Pathogenicity/Drug resistance分类
农业科技引用本文复制引用
李佳华,刘海月,李欣南,廖阳,孟凡奇,周大勇,张利勃,王辉暖..狐源肺炎克雷伯菌辽宁分离株的鉴定及其致病性与耐药性分析[J].现代畜牧兽医,2025,(2):1-5,5.基金项目
辽宁省农村科技特派行动计划(2024JH5/10400046、2024JH5/10400039) （2024JH5/10400046、2024JH5/10400039）
畜牧兽医学院科研入库项目及培育项目(2023py07) （2023py07）
