现代畜牧兽医Issue(2)：15-20,6.DOI:10.20154/j.cnki.issn1672-9692.2025.02.004
基于NGS技术的4种绵羊品种微卫星标记开发与鉴定
Development and identification of microsatellite loci in four sheep breeds based on NGS technology
摘要
Abstract
The study aimed to develop microsatellite markers in the sheep genome to provide effective molecular markers for the conservation of sheep breeds and genetic research.Through next-generation sequencing,24 microsatellite markers were developed for four indigenous Chinese sheep breeds(Lanzhou fat-tailed sheep,Tan sheep,Tibetan sheep,and small-tailed han sheep).These 24 markers were used to screen 11 Lanzhou fat-tailed sheep,19 Tan sheep,30 Tibetan sheep,and 12 small-tailed han sheep collected from Gansu Province.The results showed that in the four sheep breeds,the number of alleles per locus ranged from 2 to 11(with an average of 4).After Bonferroni correction,several loci significantly deviated from Hardy-Weinberg equilibrium(2 in Lanzhou fat-tailed sheep,9 in Tan sheep,7 in Tibetan sheep,and 2 in Xsmall-tailed han sheep),which may be the result of non-random mating.The study demonstrated that the developed markers were accurate and effective,with high polymorphism,and could be used for genetic research and genetic management of indigenous Chinese sheep breeds.关键词
微卫星/兰州大尾羊/滩羊/藏羊/小尾寒羊/绵羊保护品种Key words
Microsatellites/Lanzhou fat-tailed sheep/Tan sheep/Tibetan sheep/Small-tailed han sheep/Protected sheep breeds分类
农业科技引用本文复制引用
陈天莹,海春花,张俊哲,颜海藩,李铀..基于NGS技术的4种绵羊品种微卫星标记开发与鉴定[J].现代畜牧兽医,2025,(2):15-20,6.基金项目
国家自然科学基金资助项目(32060311) （32060311）
甘肃省委组织部省级人才计划项目(51202506) （51202506）
兰州市城关区科技局科技人才创新创业项目(2023-rc-6) （2023-rc-6）
西北民大中央高校基本科研业务费(31920240109) （31920240109）
