现代畜牧兽医Issue(2)：21-26,6.DOI:10.20154/j.cnki.issn1672-9692.2025.02.005
丹尼链球菌检测方法及其表型的研究
Research on detection methods and phenotypic characteristics of Streptococcus danieliae
摘要
Abstract
The experiment aims to develop an identification method for Streptococcus danieliae and to study its drug resistance and susceptibility characteristics.The experiment first involved the isolation and culture of the standard strain of Streptococcus danieliae CCUG57647(Sd57647),Gram staining and microscopic examination,biochemical identification,and 16S rRNA sequence analysis to determine its characteristics and establish detection methods.This was followed by verification of the detection method using five isolates separated from the tracheal secretions of SPF mice.One of the isolates Sd001,was observed for its morphological characteristics under a transmission electron microscope and subjected to a salt tolerance test.Subsequently,it was cultured up to the 20th generation on Columbia blood agar plates to examine its growth stability.The results showed that Streptococcus danieliae is a Gram-positive streptococcus with a visible capsule structure under the transmission electron microscope.On Columbia blood agar,it forms circular,smooth,semi-transparent,pale green colonies with α-hemolysis(1～2 mm in diameter)after cultivation at 36℃for 24～48 hours.Fifteen biochemical reactions with specific identification characteristics for Streptococcus danieliae were selected by comparing the biochemical identification results with those of Streptococcus thoraltensi(St001),and the results of these 15 biochemical identification reactions in the isolates from the tracheal secretions of SPF mice were consistent with those of Sd57647.The 16S rRNA phylogenetic tree analysis showed that the isolates clustered with Streptococcus danieliae.After 20 generations of subculturing,the isolates maintained stable morphological structures and biochemical reactions,but their drug susceptibility changed.The study established a detection method for Streptococcus danieliae and preliminarily investigated its phenotypic characteristics,laying the foundation for the quality control of laboratory animals.关键词
丹尼链球菌/16S rRNA/生理生化特征/生长特性/药物敏感性Key words
Streptococcus danieliae/16S rRNA/Physiological and biochemical characteristics/Growth characteristics/Antibiotic sensitivity分类
农业科技引用本文复制引用
朱琦,王昱杰,武彩红,冯丽萍..丹尼链球菌检测方法及其表型的研究[J].现代畜牧兽医,2025,(2):21-26,6.基金项目
上海实验动物研究中心新星项目(2023NS02) （2023NS02）
