河南科技大学学报（自然科学版）2025，Vol.46Issue(1)：94-104,11.DOI:10.15926/j.cnki.issn1672-6871.2025.01.012
A/O工艺不同处理阶段的生活污水微生物多样性分析
Analysis of Microbial Diversity of Domestic Wastewater at Different Treatment Stages of the A/O Process
摘要
Abstract
Revealing the diversity of wastewater microbial communities within the A/O process system can provide data support for the regulation of the process,which is conducive to the improvement of wastewater treatment efficacy.In this study,the macro-genome sequencing of the wastewater from anoxic tank,aerobic tank and sedimentation tank was performed in a domestic wastewater treatment unit of A/O process in Shuangliu District,Chengdu City,for example,and microorganisms of 213 phyla and 4 477 genera were identified.Through the analysis of the composition of the wastewater flora,it was found that the microbial community composition of the anoxic and aerobic tank wastewater was relatively similar in structure,while there was a significant difference with the sedimentation tank wastewater,and the dominant phylum of the three water samples was Pseudomonadota,with the relative abundance of 46.73%,46.50%,and 46.91%,respectively,followed by Bacteroidota,with the relative abundance of 18.11%,18.03%and 12.45%,and at the genus level,the main dominant genera in both anoxic and aerobic tank wastewater were Nitrospira and Ferruginibacter,while Dechloromonas and Azonexus became the main dominant genera in sedimentation tank wastewater.Functional genes analysis revealed that 23 genes involved in the nitrogen cycling pathway were mainly narG,nirKS and hao,and most of these genes had significantly higher abundance in anoxic and aerobic tank wastewater than in sedimentation tank.The main genera associated with carbohydrate-activating enzymes in the wastewater were Nitrospira,Ferruginibacter,Dechloromonas,Pirellula,Nitrosomonas and Azonexus,which were also the main denitrifying genera,and the enhancement of these genera may help to synchronize the improvement of carbon and nitrogen metabolism of the A/O process.In addition,Pseudomonadota,Actinomycetota,Bacteroidota,Nitrospirota,and Myxococcota are closely related to antibiotic resistance genes,and future research on enhancing the removal of antibiotic resistance genes in the A/O process can be more focused on these groups of microorganisms.The research on improving the antibiotic resistance gene removal ability of A/O process in the future can be started from these microorganisms.关键词
A/O工艺/生活污水/宏基因组/微生物多样性Key words
A/O process/domestic wastewater/macro-genome/microbial diversity分类
环境科学引用本文复制引用
孔德起,胥前,丁秋菊,谭茹怡,肖双印,何毅,曹梓瑞,卫佳欣,邵欢欢..A/O工艺不同处理阶段的生活污水微生物多样性分析[J].河南科技大学学报（自然科学版）,2025,46(1):94-104,11.基金项目
国家自然科学基金项目(51803142) （51803142）
四川省科技厅科研院所科技成果转化项目(2023JDZH0025) （2023JDZH0025）
