内蒙古民族大学学报（自然科学版）2025，Vol.40Issue(2)：23-30,8.DOI:10.14045/j.cnki.15-1220.2025.02.004
响应面法优化酸溶碱沉提取骆驼蒿总生物碱的研究
Study on the Optimization of Acid-Solution and Alkali-Isolation Extraction of Total Alkaloids from Peganum nigellastrum Bunge by Response Surface Method
摘要
Abstract
This study aims to optimize the extraction process of total alkaloids from Peganum nigellastrum Bunge,taking the extraction rate of total alkaloids from Peganum nigellastrum Bunge as the evaluation index,the methods of cation exchange resin extraction,acid-solution and alkali-isolation extraction,and organic solvent extraction were compared,and the optimal extraction method was determined to be acid-solution and alkali-isolation extraction.Single factor experiments combined with response surface method were used to optimize the acid-solution and alkali-isolation extraction process.In the single factor experiment,factors such as powder particle size,soaking time,hydrochloric acid concentration,and hydrochloric acid dosage were investigated.With the optimal value of each single factor as the central point,Box-Behnken's central combination method was used to design the factors and levels of the response surface,and optimize its extraction process conditions.The experimental results showed that when the powder particle size,soaking time,HCl concentration and HCl dosage were 60 mesh,49 h,5%and 40 mL,respectively,the extraction effect of total alkaloids from Peganum nigellastrum Bunge was the best,with a yield of 32.91%,which was close to the predicted value of the model(33.082%).Through this experimental study,a quadratic model equation was obtained that could better predict the experimental results,which laid a preliminary foundation for subsequent research on the pharmacological effects of total alkaloids from Peganum nigellastrum Bunge.关键词
骆驼蒿总生物碱/酸溶碱沉法/响应面法/提取工艺Key words
total alkaloids from Peganum nigellastrum Bunge/acid-solution and alkali-isolation method/response surface method/extraction process分类
药学引用本文复制引用
吴丽丽,王青虎,白硕雅,张朝鲁孟,吴苏要乐,美塔力..响应面法优化酸溶碱沉提取骆驼蒿总生物碱的研究[J].内蒙古民族大学学报（自然科学版）,2025,40(2):23-30,8.基金项目
内蒙古自治区卫生健康科技计划项目(202201451) （202201451）
内蒙古民族大学中药学(蒙药学)重点学科建设项目(ZYY006) （蒙药学）
蒙药研发国家地方联合工程研究中心开放基金项目(MDK2021036) （MDK2021036）
