农业环境科学学报2025，Vol.44Issue(2)：483-490,8.DOI:10.11654/jaes.2024-0219
对乙酰氨基酚对蛋白核小球藻胞外聚合物的影响
Response of extracellular polymers in Proteucotlea chlorella to acetaminophen
摘要
Abstract
To explore the different concentrations of acetaminophen(0-100 mg·L-1,AAP)on chlorophyll a(Chl-a)and extracellular polymeric substances(EPS)of Proteucotlea chlorella,and the responses of Chl-a and EPS to AAP were analyzed by sampling on days 0,2,4,6,8,10,and 12.The results showed that AAP could increase the content of chlorophyll a in Proteucotlea chlorella and promote the secretion of polysaccharides and proteins in EPS,which the change was concentration-dependent.Under the concentration of AAP with 100 mg·L-1,the content of polysaccharides and proteins in EPS increased from 223.83 and 47.02 μg·mL-1 to 361.81 and 70.12 μg·mL-1,respectively.Based on the analysis of EPS functional group changes by infrared spectroscopy,it was found that with the increase of AAP concentration,the intensity of the absorption peak representing C O increased significantly.At the same time,the absorption peaks at C O were shifted,and the absorption peaks of OH groups had a similar trend,indicating that functional groups such as OH and C O in EPS could bind to AAP.Three-dimensional fluorescence spectroscopy analysis showed that with the increase of AAP concentration,the fluorescence intensity of fulvic acids gradually decreased to disappear,and the fluorescence intensity of humic acids gradually increased,indicating that AAP could affect the composition of EPS chemical components of Proteucotlea chlorella.In conclusion,AAP can promote the synthesis of chlorophyll a and EPS in Proteucotlea chlorella algae,and the functional groups,such as fulvic acid,OH and C O in EPS,may play an important role in the binding process with AAP.关键词
对乙酰氨基酚/蛋白核小球藻/胞外聚合物/叶绿素aKey words
acetaminophen/Proteucotlea chlorella/extracellular polymers/chlorophyll a分类
资源环境引用本文复制引用
张华胜,刘月敏,王晓睿,白滔,张骁文..对乙酰氨基酚对蛋白核小球藻胞外聚合物的影响[J].农业环境科学学报,2025,44(2):483-490,8.基金项目
国家自然科学基金青年科学基金项目(51908399) （51908399）
农业农村部产地污染防控重点实验室/天津市农业环境与农产品安全重点实验室开放课题(16nybchhj-3) Young Scientists Fund of the National Natural Science Foundation of China(51908399) （16nybchhj-3）
Open Subject of Key Laboratory of Original Agro-Environmental Pollution Prevention and Control,Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Agro-Environment and Agro-product Safety(16nybchhj-3) （16nybchhj-3）
