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橡胶树三倍体品种的2n雌配子形成途径再鉴定

张源源 吴桂海 龙青姨 黄肖 高新生 李维国

热带农业科学2025,Vol.45Issue(1):1-6,6.
热带农业科学2025,Vol.45Issue(1):1-6,6.DOI:10.12008/j.issn.1009-2196.2025.01.001

橡胶树三倍体品种的2n雌配子形成途径再鉴定

Formation Pathway Reidentification of 2n Female Gametes of Hevea Triploid Varieties

张源源 1吴桂海 2龙青姨 3黄肖 1高新生 1李维国1

作者信息

  • 1. 中国热带农业科学院橡胶研究所/国家橡胶树育种中心/农业农村部橡胶树生物学与遗传资源利用重点实验室 海南 海口 571101
  • 2. 贵州医科大学生物与工程学院(健康医药现代产业学院)贵州 贵阳 550004
  • 3. 云南省热带作物科学研究所 云南景洪 666100
  • 折叠

摘要

Abstract

Rubber trees'Yunyan 77-2'and'Yunyan 77-4'are the only two triploid varieties widely planted worldwide.Pre-vious studies suggested that the 2n gametes that formed these two triploid varieties originated from first-division restitution(FDR),whereas recent studies imply that this conclusion may not be correct.To clarify the genetic pathways of the 2n gametes that form triploids and to provide a reference for using 2n gametes for polyploid breeding in Hevea brasiliensis.Low recom-bination frequency SSR markers were used for genotyping the triploid rubber tree varieties'Yunyan 77-2'and'Yunyan 77-4',and the genotypes of the 2n gametes were indirectly derived.The maternal heterozygosity of the 2n gametes was then analyzed.Eight of the ten pairs of primers displaying the"ab×cd","ab×cc"or"ab×ac"genotypes in the female and male parents were selected.According to the genotype analysis,the genotypes of the 2n gametes that formed triploids were all homozygous at eight loci,and the maternal heterozygosity of the 2n gametes was 0.The 2n gametes that form triploids'Yunyan 77-2'and'Yunyan 77-4'all originate from the female parent GT1,and the formation pathway of 2n gametes is second-division restitu-tion(SDR).

关键词

橡胶树/2n雌配子/第二次减数分裂核复原(SDR)/三倍体

Key words

Hevea brasiliensis/2n female gametes/second-division restitution(SDR)/triploid

分类

农业科技

引用本文复制引用

张源源,吴桂海,龙青姨,黄肖,高新生,李维国..橡胶树三倍体品种的2n雌配子形成途径再鉴定[J].热带农业科学,2025,45(1):1-6,6.

基金项目

海南省自然科学基金(No.321MS096) (No.321MS096)

现代农业产业技术体系(No.CARS-33-YZ2). (No.CARS-33-YZ2)

热带农业科学

1009-2196

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