热带农业科学2025,Vol.45Issue(1):1-6,6.DOI:10.12008/j.issn.1009-2196.2025.01.001
橡胶树三倍体品种的2n雌配子形成途径再鉴定
Formation Pathway Reidentification of 2n Female Gametes of Hevea Triploid Varieties
摘要
Abstract
Rubber trees'Yunyan 77-2'and'Yunyan 77-4'are the only two triploid varieties widely planted worldwide.Pre-vious studies suggested that the 2n gametes that formed these two triploid varieties originated from first-division restitution(FDR),whereas recent studies imply that this conclusion may not be correct.To clarify the genetic pathways of the 2n gametes that form triploids and to provide a reference for using 2n gametes for polyploid breeding in Hevea brasiliensis.Low recom-bination frequency SSR markers were used for genotyping the triploid rubber tree varieties'Yunyan 77-2'and'Yunyan 77-4',and the genotypes of the 2n gametes were indirectly derived.The maternal heterozygosity of the 2n gametes was then analyzed.Eight of the ten pairs of primers displaying the"ab×cd","ab×cc"or"ab×ac"genotypes in the female and male parents were selected.According to the genotype analysis,the genotypes of the 2n gametes that formed triploids were all homozygous at eight loci,and the maternal heterozygosity of the 2n gametes was 0.The 2n gametes that form triploids'Yunyan 77-2'and'Yunyan 77-4'all originate from the female parent GT1,and the formation pathway of 2n gametes is second-division restitu-tion(SDR).关键词
橡胶树/2n雌配子/第二次减数分裂核复原(SDR)/三倍体Key words
Hevea brasiliensis/2n female gametes/second-division restitution(SDR)/triploid分类
农业科技引用本文复制引用
张源源,吴桂海,龙青姨,黄肖,高新生,李维国..橡胶树三倍体品种的2n雌配子形成途径再鉴定[J].热带农业科学,2025,45(1):1-6,6.基金项目
海南省自然科学基金(No.321MS096) (No.321MS096)
现代农业产业技术体系(No.CARS-33-YZ2). (No.CARS-33-YZ2)