热带农业科学2025，Vol.45Issue(1)：63-68,6.DOI:10.12008/j.issn.1009-2196.2025.01.010
海南岛辣椒疫霉菌侵染胡椒过程的细胞学观察
Cytology Observation on Process of Black Pepper Infected by Phytophthora capsici from Hainan Island
摘要
Abstract
The Phytophthora foot rot disease caused by P.capsici was considered as the first harmful black pepper(Piper nigrum L)disease in Hainan island of China.Disease infection process is the directly interaction of"pathogen-host plant",also the necessary prerequisite for the pathogenesis mechanism study and the disease management.However,the cytological proc-ess of black pepper infected by P.capsica has not been reported yet.This study,the cytological morphology of the pathogen and host cells during"P.capsici-black pepper"interaction was observed.The main results are as follows.(1)Pre-inoculation,zoospores were spherical shaped,swimming freely after being released.(2)3 h after inoculation,zoospores attached to the hypocotyl epidermis and developing into flat spherical cysts.Most cysts further deformed into pear shaped or ellipsoidal shapes,while a few still keep spherical shape but germinated short tubes.The host cell sites attached with the further deformed cysts were induced to produce reactive oxygen species and callose.(3)6 h after inoculation,long germ tubes could be detected with swollen ends as appressorium at the host epidermis.The invasive hyphae expanded through the intercellula space of the host cells,and developed clusters of finger-shaped haustoria in cells along the expanding way.Significantly callose deposition and reactive oxygen species(ROS)burst were induced within infected host cells,and the cell death could also be detected at this point.(4)24 h after inoculation,the invasive hyphae extended to the xylem tissue of the host hypocotyl,and colonized full fill the pith cells.Invasive hyphae rapidly expanding along xylem cells in the vertical direction,whereas intercellularly ex-panding in the horizontal with a relatively slow speed.(5)48 h after inoculation,aerial hyphae sprouted up through stomata and other intercellular space of host plant.To the best of our knowledge,this is the first report of cytology process of black pepper infected by P.capsica and the induced black pepper cell defense response,would be help for further study of the"P.capsica-black pepper"interaction and the pathogenesis of the foot rot disease in black pepper.关键词
胡椒/辣椒疫霉菌/侵染过程/显微观察Key words
black pepper/Phytophthora capsici/infection process/microscopic observation分类
农业科技引用本文复制引用
高圣风,王亚男,付华菲,胡生燕,刘世超,孙世伟..海南岛辣椒疫霉菌侵染胡椒过程的细胞学观察[J].热带农业科学,2025,45(1):63-68,6.基金项目
海南自然科学基金项目(No.320MS104) （No.320MS104）
海南省重点研发计划项目(No.ZDYF2022XDNY169) （No.ZDYF2022XDNY169）
国家自然科学基金(No.31972329). （No.31972329）
