水产科技情报2025，Vol.52Issue(2)：98-107,10.DOI:10.16446/j.fsti.20240600113
大连地区刺参野生群体和养殖群体肠道菌群结构分析
Analysis of intestinal flora structure of wild and cultured Apostichopus japonicus in Dalian area
摘要
Abstract
To understand the intestinal microflora characteristics of wild and cultured populations of Apostichopus japonicus in Dalian,16S rRNA high-throughput sequencing technology was used to analyze the intestinal microflora characteristics of wild and cultured populations of A.japonicus in Dalian.Alpha diversity analysis showed that the intestinal microbial diversity and richness of cultured A.japonicus in Jinzhou District of Dalian were higher than those in wild A.japonicus in Sanshan Island area.At the phylum level,the predominant phyla in the intestinal flora of cultured A.japonicus were Proteobacteria,Desulfobacterota,Bacteroidetes,and Planctomycetes with the relative abundance of 54.02%,18.90%,7.45%and 6.02%,respectively.However,the predominant phyla in the intestinal flora of wild A.japonicus were Proteobacteria,Bacteroidetes,and Verrucomicrobia with the relative abundance of 54.69%,28.08%and 11.89%,respectively.The proportion of Desulfobacterota and Planctomyce-tes in the intestinal microflora of cultured A.japonicus was 18.90%and 6.02%,respectively,but these two phyla in wild A.japonicus accounted for less than 0.5%.At the genus level,norank Desulfocapsaceae,norank Cau-lobacteraceae,and Ralstonia were the dominant genera in the intestinal flora of cultured A.japonicus,with a rela-tive abundance of 17.26%,8.55%and 7.06%,respectively.Sedimentitalea,Lutibacter,Persicirhabdus,and Leucothrix were the dominant genera in the intestinal flora of wild A.japonicus,with a relative abundance of 23.52%,18.53%,8.73%and 8.37%,respectively.Sedimentitalea,Lutibacter,Persicirhabdus,and Leucothrix accounted for 8%-24%in the intestinal microflora of wild A.japonicus,but less than 0.5%in the cultured A.japonicus.Norank Desulfocapsaceae,norank Caulobacteraceae,and Ralstonia accounted for 7%-18%in the intestinal microflora of cultured A.japonicus,but less than 0.1%in the wild A.japonicus.The results showed that different environments had a significant impact on the composition of the intestinal microorganisms of A.japonicus,and there was spatial heterogeneity in the composition of the intestinal microorganisms.关键词
刺参/高通量测序/肠道微生物/多样性Key words
Apostichopus japonicus/high-throughput sequencing/intestinal microorganisms/diversity引用本文复制引用
刘丹,潘浩然,赵新延,汪南壹,许天浩,丁君,常亚青,田燚..大连地区刺参野生群体和养殖群体肠道菌群结构分析[J].水产科技情报,2025,52(2):98-107,10.基金项目
海参、海胆种质创制技术研发与新品种培育(20230023). （20230023）
