钱璟 1梅亚洁 1郭琳 2吴思念 1史明艳 1陈万光1
作者信息
- 1. 洛阳师范学院,河南省冷水鱼养殖工程技术研究中心,河南省豫西地区黄河湿地生态系统野外科学观测研究站,河南 洛阳 471934
- 2. 伊川思源实验学校,河南 洛阳 471399
- 折叠
摘要
Abstract
To investigate the tissue distribution and activity changes of non-specific immune factors in Chinese giant salamander(Andrias davidianus),the contents of nitric oxide(NO),total nitric oxide synthase(TNOS),inducible nitric oxide synthase(iNOS),lysozyme(LYZ),acid phosphatase(ACP),alkaline phosphatase(AKP),complement C3(C3),complement C4(C4)and transferrin(Tf)were determined in the liver,intestine,and spleen of two age groups of A.davidianus using chemical colorimetric methods and enzyme-linked immunosorbent assay(ELISA).The results showed that the nine non-specific immune factors were distributed in all these three tissues,while their activities varied between different age groups and tissues.In the one-year-old A.davidianus,the activities of LYZ,ACP,AKP and Tf in the spleen were higher than those in the liver and intestine.The activities of NO,C3 and C4 in the liver were higher than those in the spleen and intestine,while the activities of TNOS and iNOS in the intestine were higher than those in the spleen and liver.In the two-year-old A.davidianus,the activities of NO,TNOS,iNOS,C3 and C4 in the liver were higher than those in the spleen and intestine.The activities of LYZ and AKP in the intestine were higher than those in the liver and spleen,while the activities of ACP and Tf in the spleen were higher than those in the liver and intestine.The results of this study indicated that the spleen and the liver were the main distribution areas of these non-specific immune factors in the one-year-old A.davidianus,while the liver was the main distribution area of these non-specific immune fac-tors in two-year-old A.davidianus.关键词
大鲵/非特异性免疫因子/免疫组织/组织分布/活性变化Key words
Andrias davidianus/non-specific immune factor/immune tissue/tissue distribution/activity change
