水产科技情报2025，Vol.52Issue(2)：116-122,7.DOI:10.16446/j.fsti.20240300101
低盐度下亚硝酸盐对凡纳滨对虾毒性与抗氧化能力的影响
Effects of nitrite exposure on the toxicity and antioxidant capability of Litopenaeus vannamei at low salinity
摘要
Abstract
To explore the effects of nitrite on the acute toxicity and oxidative stress of Litopenaeus vannamei under low salinity conditions,an acute toxicity test of nitrite on L.vannamei was conducted in water with salinity of 1.The an-tioxidant indexes of shrimp were measured during nitrite exposure(4 mg/L,8 mg/L)and recovery periods.The sur-vival rate,molting rate,and feeding time of shrimps were recorded after 24 h recovery.The results showed that LC50(median lethal concentration)of nitrite for L.vannamei at 24,48,72 and 96 h were 15.96,9.97,7.54,and 5.25 mg/L at a salinity of 1,respectively,and the safe concentration(SC)was 0.53 mg/L.The activity of superoxide dis-mutase(SOD)in the hemolymph of shrimp increased after exposure to different concentrations of nitrite,and was sig-nificantly higher than that before exposure to nitrite(P<0.05).The SOD activity in the hepatopancreas of shrimp trea-ted with 8 mg/L nitrite for 3 and 6 h was significantly higher than that in the 4 mg/L group(P<0.05).The glutathione peroxidase(GSH-Px)activity in hemolymph and hepatopancreas also increased,and GSH-Px activity in the 8 mg/L nitrite treatment group was significantly higher than that in the 4 mg/L group for 6 h(P<0.05).The catalase(CAT)activity in the hemolymph decreased after 4 mg/L nitrite treatment for 6 and 24 h,and it was significantly lower than that before treatment(P<0.05).The CAT activity in the hepatopancreas fluctuated and was significantly higher after 24 h treatment(P<0.05).The CAT activity in the shrimp with 8 mg/L nitrite treatment for 24 h was significantly higher than that in the 4 mg/L group(P<0.05).After being transferred to fresh culture water without adding nitrite for 24 h(F24),the malondialdehyde(MDA)content in the hepatopancreas was still significantly higher than that without nitrite treatment(P<0.05).MDA content in 8 mg/L nitrite treatment group was significantly higher than that in the 4 mg/L group(P<0.05).Compared with the control group with fresh culture water,the survival rate of shrimps in 4 and 8 mg/L groups(F24)showed a decrease,the molting rate was significantly increased and the feeding time was signifi-cantly extended(P<0.05).The survival rate in the 8 mg/L group(F24)was significantly lower and the feeding time was significantly longer compared to the 4 mg/L group(F24)(P<0.05).The results indicated that nitrite toxicity was stronger in low salinity aquaculture water,which significantly affected the antioxidant capacity of shrimps.关键词
亚硝酸盐/凡纳滨对虾/低盐度/毒性/抗氧化能力Key words
nitrite/Litopenaeus vannamei/low salinity/toxicity/antioxidant ability引用本文复制引用
王澳林,姜玉声,邢燕露,王成杰,焦宁,孙娜..低盐度下亚硝酸盐对凡纳滨对虾毒性与抗氧化能力的影响[J].水产科技情报,2025,52(2):116-122,7.基金项目
国家重点研发计划"政府间国际科技创新合作"项目(2022YFE0117900) （2022YFE0117900）
2023辽宁省科技重大专项种质创新工程(2024JH1/11700009) （2024JH1/11700009）
辽宁省属本科高校基本科研业务费专项资金资助(LJ222410158050) （LJ222410158050）
2023年大学生创新创业训练计划项目. （）
