四川中医2025，Vol.43Issue(2)：105-111,7.
基于miR-451a/p38MAPK通路研究柴胡清肝汤干预肉芽肿性小叶性乳腺炎大鼠的机制
Mechanism study of Chaihu Qinggan Decoction intervention in granulomatous lobular mastitis rats based on miR-451a/p38MAPK pathway/
摘要
Abstract
Objective:Investigate the regulatory impact of Chaihu Qinggan Decoction on the miR-451a/p38MAPK pathway in granulomatous lobular mastitis(GLM)rats.Methods:A GLM rat model was established,and 60rats were randomly divided into normal group,model group,low-,medium-,high-dose groups of traditional Chinese medicine,and hormone group.The breast inflammation index was calculated,and the histopathological changes of the breast were observed by HE staining.Western blot and qRT-PCR were used to detect the expression levels of MKK3,p-p38MAPK,p38MAPK protein and miR-451a,p38MAPK,MKK3mRNA in breast tissue.Serum CRP,IL-8and IL-2levels were detected by ELISA.Results:Compared with the normal group,the mammary inflammation index of the model group was significantly increased(P<0.01),the structure of granuloma was pathologically formed with a variety of inflammatory cell infiltration,the expression of miR-451a,MKK3,p-p38MAPK and p38MAPK in mammary tissue was increased(P<0.01),and the contents of IL-2,IL-8and CRP in serum were increased(P<0.01).Compared with the model group,the middle-dose and high-dose groups and the hormone group could re-duce the breast inflammation index(P<0.01),alleviate the histopathological damage and inflammatory cell infiltration of the breast tissue,reduce the expression levels of miR-451a,MKK3,p-p38MAPK and p38MAPK in breast tissue(P<0.05,P<0.01),and reduce the serum IL-2,IL-8and CRP contents(P<0.01).Conclusion:Chaihu Qinggan Decoction may inhibit the release of inflammatory factors and reduce inflammatory damage by regulating the miR-451a/p38MAPK pathway,thereby ex-erting a therapeutic effect of inhibiting GLM inflammatory response.关键词
肉芽肿性小叶性乳腺炎/miR-451a/p38MAPK/柴胡清肝汤/炎症因子Key words
Granulomatous lobular mastitis/miR-451a/p38MAPK/Chaihu Qinggan Decoction/Inflammatory factors分类
医药卫生引用本文复制引用
苏靖群,凌洁,李恋,熊帆,丁笑,刘清,王华,刘丽芳..基于miR-451a/p38MAPK通路研究柴胡清肝汤干预肉芽肿性小叶性乳腺炎大鼠的机制[J].四川中医,2025,43(2):105-111,7.基金项目
湖南省中医药科研计划项目(编号:202100) （编号:202100）
湖南省中医药管理局"十四五"第一批中医药领军人才培养项目(编号:rsk-023～02) （编号:rsk-023～02）
湖南中医药大学校级科研基金(编号:2022CX139、2023CX91). （编号:2022CX139、2023CX91）
