山东农业大学学报（自然科学版）2025，Vol.56Issue(1)：83-92,10.DOI:10.3969/j.issn.1000-2324.2025.01.009
牛蒡种子不同萌发阶段内生菌多样性、功能及牛蒡炭疽病害防控研究
Diversity and Function of Endophytes in Different Germination Stages of Arctium lappa Seeds and Their Roles in Prevention and Control of A.lappa Anthracnose Disease
摘要
Abstract
This study was conducted to isolate,purify,and conduct molecular identification,functional assays,antagonistic effects,as well as analyze the changes in arctiin and arctigenin contents in different germination stages of Arctium lappa seeds,including dry seeds,seeds after 24-hour water absorption,seeds that did not germinate after 4-day culture,seedlings,and non-germinating seeds.Initially,21 culturable endophytic strains were successfully isolated and purified by the streak plate method,with Enterobacteriaceae being the predominant bacterial group.Using molecular biological techniques,the taxonomic status and species diversity of these endophytes were clarified.Furthermore,the functional exploration of these endophytes were conducted through experiments on nitrogen fixation,phosphate solubilization,siderophore production,and IAA synthesis.HPLC was used to analyze the influence of these endophytic on the arctiin and arctigenin content in A.lappa seeds across different germination stages.The content of arctiin and arctigenin in A.lappa seeds at different germination stages was analyzed using High-Performance Liquid Chromatography(HPLC)to assess the influence of endophytes.Meanwhile,the antagonistic effects of the endophytes against the anthracnose pathogen in A.lappa anthracnose were evaluated using the plate confrontation method,and strains with significant antagonistic capabilities were screened out.Finally,the germination experiment of treating seeds with bacterial suspension verified the promoting effect of these endophytes on the growth of A.lappa,and improved the germination rate and germination potential of seeds.This research provides scientific basis and practical guidance for the development and utilization of A.lappa endophytic resources,and the sustainable development of traditional Chinese medicine resources.关键词
牛蒡/种子萌发/内生菌/促生功能Key words
Arctium lappa/seed germination/endophytes/growth-promoting function分类
生物学引用本文复制引用
于莹,高铭泽,薛嘉宁,赵容..牛蒡种子不同萌发阶段内生菌多样性、功能及牛蒡炭疽病害防控研究[J].山东农业大学学报（自然科学版）,2025,56(1):83-92,10.基金项目
国家自然科学基金(81874338) （81874338）
