山东农业大学学报（自然科学版）2025，Vol.56Issue(1)：125-132,8.DOI:10.3969/j.issn.1000-2324.2025.01.014
拟南芥根中细胞器特异标记蛋白质的定位观察
Localization of Organelle-specific Marker Proteins in Arabidopsis thaliana Roots
摘要
Abstract
The tiny structures or compartments that make up a cell are collectively referred to as subcellular structures.Each subcellular structure contains specific proteins.The function of unknown proteins can be inferred through co-localization techniques with these specific proteins.The distribution and morphology observation of each organelle in co-localization technology requires corresponding organelle markers to indicate.However,there are few systematic and comprehensive reports on the localization of organelle-specific marker proteins in the literature.In this paper,we observed the localization of organelle-specific marker proteins in various organelles through confocal microscopy and recorded the distribution of each organelle in the cell,A further differitiention was made among the plesiomorphic Organelles with chemical treatment and super-resolution imaging.This paper was aimed to provide a reference for the localization of unknown proteins in subsequent studies.关键词
细胞器特异标记蛋白质/亚细胞定位/细胞器/共聚焦显微镜Key words
Organelle-specific marker proteins/subcellular localization/organelles/confocal microscope分类
生物科学引用本文复制引用
徐萌,王亚楠,李婷婷,赵新英..拟南芥根中细胞器特异标记蛋白质的定位观察[J].山东农业大学学报（自然科学版）,2025,56(1):125-132,8.基金项目
拟南芥RAB5调控花粉管生长的功能和机理研究(32100296) （32100296）
