山东农业大学学报（自然科学版）2025，Vol.56Issue(1)：141-150,10.DOI:10.3969/j.issn.1000-2324.2025.01.016
四种天然抑菌物质对单增李斯特菌生物膜形成的影响
Four Natural Antimicrobial Substances on Biofilm Formation of Listeria monocytogenes
摘要
Abstract
To investigate the biofilm inhibitory effects of natural antimicrobial substances of Listeria monocytogenes(Lm),our study first determined the minimal inhibitory concentrations(MIC)and sub-inhibitory concentrations(SIC)of the natural antimicrobial substances cinnamaldehyde,eugenol,resveratrol,and thymoquinone against Lm ATCC 19115.The effects of four natural antibacterial substances on the biofilm formation ability of the strain were studied by measuring the swimming ability,biofilm metabolic activity(CCK-8 method)and scanning electron microscopy at different concentrations(MIC,1/4 MIC and 1/8 MIC).The results showed that the MICs of cinnamaldehyde,eugenol,resveratrol,and thymoquinone against Lm ATCC 19115 were 640,1 280,400,and 50 μg/mL,respectively.The SICs of Lm ATCC 19115 for the four natural antimicrobial substances were 1/4 MIC and 1/8 MIC.All of the above 4 natural antimicrobial substances were found to inhibit the biofilm formation of Lm ATCC 19115,with inhibition rates exceeding 50%even at the sub-inhibitory concentration of 1/4 MIC.Among the four natural antimicrobial substances,cinnamaldehyde exhibited the best biofilm inhibition effect,it effectively inhibited the biofilm formation of Lm ATCC 19115 by inhibiting its swimming motility and biofilm metabolic activity.关键词
单增李斯特菌/生物膜/天然抑菌物质/亚抑菌浓度Key words
Listeria monocytogenes/biofilm/natural antimicrobial substances/sub-inhibitory concentration分类
轻工纺织引用本文复制引用
孙立龙,罗欣,朱立贤,董鹏程,韩广星,仝林,张一敏,刘昀阁..四种天然抑菌物质对单增李斯特菌生物膜形成的影响[J].山东农业大学学报（自然科学版）,2025,56(1):141-150,10.基金项目
国家自然科学基金(32302181) （32302181）
山东省自然科学基金(ZR2022QC182) （ZR2022QC182）
国家肉牛牦牛产业技术体系(CARS-37) （CARS-37）
山东省牛产业创新团队(SDAIT-09-09) （SDAIT-09-09）
