药学研究2025，Vol.44Issue(2)：113-117,5.DOI:10.13506/j.cnki.jpr.2025.02.002
片仔癀对MCAO大鼠血脑屏障通透性及AMPK/PKC通路的影响
Effects of Pien Tze Huang on blood brain barrier permeability and AMPK/PKC pathway in MCAO rats
摘要
Abstract
Objective To investigate the effects of Pien Tze Huang(PTH)on blood-brain barrier damage and related regulatory proteins in rats with cerebral ischemia/reperfusion injury.Methods Healthy adult male Sprague-Dawley rats were randomly divided into sham operation group,model group and PTH group.Rats were orally administered PTH for 4 days before modeling,and the last dose was given 30 minutes before middle cerebral artery occlusion(MCAO)using the suture method.Zea-longa neurofunctional score was used to evaluate the protective effect of PTH on MCAO rats.Evans blue staining method was used to detect the changes in BBB permeability of cerebral ischemia.The expression of Occludin,Claudin-5,zonula occluden-1(ZO-1),matrix metalloproteinases-9(MMP-9),protein kinase C(PKC),adenine monophosphate activated protein kinase(AMPK)and its phosphorylated proteins(p-AMPK)in MCAO rats were determined by Western blotting.Results Compared with the model group,PTH significantly improved the neurological deficit(P＜0.05),decreased BBB permeability(P＜0.05),promoted Occludin,Claudin-5,ZO-1 and PKC protein expression(P＜0.01),decreased MMP-9 protein expression(P＜0.01),inhibited AMPK phosphorylation(P＜0.05).Conclusion Pien Tze Huang could regulate the expression of tight junction protein and PKC protein,reduce the expression of MMP-9 protein,inhibit the activation of AMPK protein,repair the blood-brain barrier,and reduce brain injury.关键词
片仔癀/脑缺血/再灌注/血脑屏障/紧密连接蛋白/腺苷酸活化蛋白激酶/蛋白激酶CKey words
Pien Tze Huang/Cerebral ischemia/reperfusion/Blood-brain barrier/Tight junction proteins/AMPK/PKC分类
中医学引用本文复制引用
张小琴,林钰欣,韩美婷,黄莉莉,吴珍琴,黄鸣清..片仔癀对MCAO大鼠血脑屏障通透性及AMPK/PKC通路的影响[J].药学研究,2025,44(2):113-117,5.基金项目
国家自然科学基金项目(No.81973437、82274080) （No.81973437、82274080）
福建省自然科学基金项目(No.2022J01869、2023J011565) （No.2022J01869、2023J011565）
福建中医药大学校管项目(No.X2021002) （No.X2021002）
