药学研究2025，Vol.44Issue(2)：123-126,4.DOI:10.13506/j.cnki.jpr.2025.02.004
电阻法测定人乳头瘤病毒病毒样颗粒的粒径
Determination of particle size of virus-like particle of HPV by resistance pulse sensor method
摘要
Abstract
Objective To establish a method for determining the particle size of virus like particles using the Resistance Pulse Sensor(RPS)method.Methods The NanoCoulter was used to measure the particle size of the human papillomavirus(HPV)vaccine stock solution.Methodological validation was conducted on accuracy,precision and repeatability,and the particle size detection results were compared with negative staining electron microscopy results.Results The average particle size of 100 nm standard microspheres was(102.00±0.00)nm,and the result of 6 measurements was(102.33±0.52)nm.The sample was measured 6 times and the result was(78.67±1.37)nm.This indicates that the method has good accuracy,precision,and repeatability.The sample measurement results are comparable to the negative staining electron microscopy results.The sample was left at 56℃for 30 minutes,with the highest number of large particles.Conclusion The resistance method can be used for particle size detection distribution and stability monitoring of virus like particle products.关键词
库尔特电阻法/人乳头瘤病毒/粒径与粒径分布/病毒样颗粒Key words
Coulter resistance method/HPV/Particle size and particle size distribution/Virus like particles分类
药学引用本文复制引用
路琼,纳涛,黄维金,聂建辉..电阻法测定人乳头瘤病毒病毒样颗粒的粒径[J].药学研究,2025,44(2):123-126,4.基金项目
国家重点研发计划项目(No.2021YFC2302505) （No.2021YFC2302505）
