药学研究2025，Vol.44Issue(2)：165-169,5.DOI:10.13506/j.cnki.jpr.2025.02.011
柱后衍生阳离子交换色谱法测定依降钙素中氨基酸组成与肽含量
Determination of amino acid composition and peptide content in elcatonin by post-column derivatization cation-exchange chromatography
摘要
Abstract
Objective To determine the amino acid composition and peptide content in elcatonin using amino acid analyzer and compare the results with those obtained from the pre-column derivatization high-performance liquid chromatography(HPLC)method.Methods The chromatographic column was 2622SC-PH(4.6 mm×60 mm).The flow rate of pump A(pH series solution)was 0.4 mL·min-1,and the flow rate of pump B(ninhydrin solution)was 0.35 mL·min-1.The detection wavelengths were set at 440 nm(proline)and 570 nm(other amino acids).Results The results showed that the linear relationship of the 14 amino acids was good,the repeatability RSD was 1.1%～2.5%,and the recovery was 98.0%-103.6%.The results were generally consistent with those obtained from the HPLC method.Conclusion The amino acid analyzer method was simple and highly automated,and can be used to determine the amino acid composition and peptide content in elcatonin.关键词
依降钙素/柱后衍生/阳离子交换色谱/氨基酸/肽Key words
Elcatonin/Post-column derivatization/Cation-exchange chromatography/Amino acids/Peptides分类
医药卫生引用本文复制引用
薛维丽,梁超超,石峰,巩丽萍,张冬梅,咸瑞卿..柱后衍生阳离子交换色谱法测定依降钙素中氨基酸组成与肽含量[J].药学研究,2025,44(2):165-169,5.基金项目
山东省重点研发计划项目(No.2022TZXD004) （No.2022TZXD004）
泰山产业领军人才工程项目(No.tscx202306076) （No.tscx202306076）
