实验动物与比较医学2025，Vol.45Issue(1)：13-20,8.DOI:10.12300/j.issn.1674-5817.2024.100
SHJHhr小鼠的心脏衰老表型研究
Study on Cardiac Aging Phenotypes of SHJHhrMice
摘要
Abstract
Objective To investigate the spontaneous premature cardiac aging in SHJHhr mice.Methods A comparative study was conducted between SHJHhr mice(SHJHhr group)and wild-type ICR mice(WT group)at different ages(10 and 24 weeks).Cardiac function was analyzed using a small animal in vivo ultrasound imaging system.After euthanasia,organs were collected and weighed to assess the extent of cardiac atrophy.Cardiac pathological damage was observed using hematoxylin-eosin(HE)staining.Cardiac fibrosis was analyzed using Masson staining.Myocardial cell area was analyzed after wheat germ agglutinin(WGA)staining.The activities of oxidative damage indicators in myocardial tissue,including superoxide dismutase(SOD),glutathione peroxidase(GPX),and catalase(CAT),as well as the content of 8-hydroxy-2'-deoxyguanosine(8-OHdG),were measured using enzyme-linked immunosorbent assay.Real-time fluorescence quantitative PCR was used to measure the mRNA expression levels of factors associated with inflammation,fibrosis,and oxidative stress.Colorimetric assay was used to measure malondialdehyde(MDA)levels.Results Compared to WT group mice of the same age,10-week-old mice in the SHJHhrgroup showed no significant differences in stroke volume(SV),ejection fraction(EF),fractional shortening(FS),or heart and lung weights.However,at 24 weeks of age,mice in the SHJHhr group had significantly lower SV,EF,and FS values compared to mice of the same age in the WT group(P＜0.05),with no significant change in lung weight but a significant reduction in heart weight(P＜0.05).Histological analysis of heart tissue from 24-week-old mice revealed no significant difference in cardiac fibrosis levels between SHJHhr and WT groups,but WGA staining showed a significant reduction in myocardial cell area in mice in the SHJHhr group(P＜0.05).PCR analysis revealed a significant downregulation of mRNA levels of oxidative stress factors Sod2,Gpx1,and Cat genes(P＜0.05).Biochemical assays indicated significantly reduced activities of oxidative damage-related enzymes SOD,GPX,and CAT in myocardial tissue(P＜0.05),while the levels of oxidative damage markers 8-OHdG and MDA significantly increased(P＜0.05).Conclusion Mice in the SHJHhr group exhibit premature cardiac aging,which may be associated with oxidative stress damage in myocardial tissue.关键词
SHJHhr小鼠/心脏衰老/氧化损伤Key words
SHJHhrmice/Cardiac aging/Oxidative damage引用本文复制引用
刘荣乐,程灏,尚付生,常书福,徐平..SHJHhr小鼠的心脏衰老表型研究[J].实验动物与比较医学,2025,45(1):13-20,8.基金项目
上海市科技创新行动计划项目"自发性早衰老小鼠的心血管衰老相关表型分析及机制研究"(21140900103) （21140900103）
