实验动物与比较医学2025，Vol.45Issue(1)：101-111,11.DOI:10.12300/j.issn.1674-5817.2024.109
提高动物实验系统评价/Meta分析的证据确定性:GRADE方法的实证研究
Improving the Certainty of Evidence in Animal Experiment Systematic Review/Meta-Analysis:An Empirical Study of the GRADE Method
摘要
Abstract
Animal experiments are essential tools in biomedical research,serving as a bridge between basic research and clinical trials.Systematic reviews and meta-analyses(SRs/MAs)of animal experiments are crucial methods for integrating evidence from animal experiment,which can facilitate the translation of findings into clinical research,reduce translational risks,and promote resource integration in basic research.With the continuous development of the Grading of Recommendations,Assessment,Development,and Evaluation(GRADE)methodology,its application in SRs/MAs of animal experiments has gained increasing attention.This article first outlines the principles and specific applications of the GRADE methodology in SRs/MAs of animal experiments,including qualitative descriptive systematic reviews,meta-analyses,and network meta-analyses.It then deeply analyzes the misuse of the GRADE methodology in practice,including incorrect evidence grading,improper classification of evidence,misapplication in qualitative systematic reviews,inconsistencies between the documentation of the upgrading and downgrading process and results,and inappropriate use for making recommendations.Furthermore,this article comprehensively discusses the factors influencing the grading of evidence certainty in SRs/MAs of animal experiments,including the impact of bias risk,indirectness,inconsistency,imprecision,and publication bias on evidence downgrading,as well as the role of large effect sizes and cross-species consistency in evidence upgrading.Finally,in response to the issues discussed,improvement strategies are proposed,including further research and optimization of the GRADE methodology for SRs/MAs of animal experiments,the development of reporting guidelines tailored to the characteristics of SRs/MAs in animal experiment research,and enhanced professional training for researchers in the GRADE methodology.This article aims to improve the quality of evidence in SRs/MAs of animal experiments,strengthen their reliability in clinical decision-making,and promote the more efficient translation of findings from animal experiment research into clinical practice.关键词
动物实验/系统评价/Meta分析/推荐分级的评估、制订与评价/证据确信度/证据分级Key words
Animal experiment/Systematic reviews/Meta-analyses/Grading of recommendations assessment,development and evaluation/Certainty of evidence/Evidence grading分类
生物学引用本文复制引用
李腾飞,田金徽,郑卿勇,许建国,李艺羿,周泳佳,徐彩花,张明悦,田杰祥,王钢..提高动物实验系统评价/Meta分析的证据确定性:GRADE方法的实证研究[J].实验动物与比较医学,2025,45(1):101-111,11.基金项目
2023年中央财政转移支付地方项目"中医药循证能力提升建设"(2101704) （2101704）
