上海针灸杂志2025，Vol.44Issue(3)：347-354,8.DOI:10.13460/j.issn.1005-0957.2024.13.4005
基于HMGB1-RAGE信号通路探究川芎嗪结合电针对阿尔茨海默症大鼠的神经保护作用
Study of the neuroprotective effect of Tetramethylpyrazine combined with electroacupuncture on Alzheimer's disease rats based on the HMGB1-RAGE signaling pathway
摘要
Abstract
Objective To explore the neuroprotective effect of Tetramethylpyrazine plus electroacupuncture(EA)on Alzheimer's disease(AD)rats and its effect on the high mobility group protein 1(HMGB1)/advanced glycosylation end-product receptor(RAGE)signaling pathway.Method AD rat models were established.The experimental rats were divided into a control group,a model group,a Tetramethylpyrazine group,an EA group,a Tetramethylpyrazine+EA group,and a Tetramethylpyrazine+EA+HMGB1 group.The Morris water maze test was used to assess the rat's learning,memory,and spatial exploration abilities;the enzyme-linked immunosorbent assay was adopted to detect the levels of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in the rat's hippocampal;hematoxylin-eosin(HE)staining was employed to observe morphological changes in the hippocampal tissue;immunochemistry was used to check the protein expression of beta-amyloid 1-42(Aβ1-42)and phosphorylated Tau(p-Tau);the protein expression of HMGB1 and RAGE was determined using Western blot(WB).Result Compared to the control group,rats in the model group showed a longer escape latency(P＜0.05),decreased platform crossings(P＜0.05),reduced platform dwell time(P＜0.05),disordered hippocampal neuron arrangement with significant loss and degeneration of neurons and pyknosis,and increased expression of TNF-α,IL-1β,Aβ1-42,p-Tau,HMGB1,and RAGE(P＜0.05).Compared to the model group,rats in the Tetramethylpyrazine and EA groups showed a shortened escape latency(P＜0.05),increased platform crossings(P＜0.05),longer platform dwell time(P＜0.05),reduced hippocampal neuron damage,and reduced expression of TNF-α,IL-1β,Aβ1-42,p-Tau,HMGB1,and RAGE(P＜0.05).Compared to the Tetramethylpyrazine and EA groups,the Tetramethylpyrazine+EA group showed a shortened escape latency(P＜0.05),increased platform crossings(P＜0.05),longer platform dwell time(P＜0.05),reduced hippocampal neuron damage,and decreased expression of TNF-α,IL-1β,Aβ1-42,p-Tau,HMGB1,and RAGE(P＜0.05).Compared to the Tetramethylpyrazine+EA group,rats in the Tetramethylpyrazine+EA+HMGB1 group showed an increase in the escape latency(P＜0.05),decreased platform crossings(P＜0.05),shorter platform dwell time(P＜0.05),aggravated hippocampal neuron damage,and increased expression of TNF-α,IL-1β,Aβ1-42,p-Tau,HMGB1,and RAGE(P＜0.05).Conclusion The combined use of Tetramethylpyrazine and EA can mitigate nerve damage in AD rats by inhibiting the HMGB1/RAGE signaling pathway.关键词
电针/针药并用/阿尔茨海默病/川芎嗪/高迁移率族蛋白1/晚期糖基化终末产物受体信号通路/大鼠Key words
Electroacupuncture therapy/Alzheimer disease/Tetramethylpyrazine/High mobility group protein 1/advanced glycosylation end-product receptor signaling pathway/Rats分类
医药卫生引用本文复制引用
何诚,王颖..基于HMGB1-RAGE信号通路探究川芎嗪结合电针对阿尔茨海默症大鼠的神经保护作用[J].上海针灸杂志,2025,44(3):347-354,8.基金项目
武汉市卫生计生委办公室中医类科研项目(WZ21Z02) （WZ21Z02）
武汉市中心医院学科基金项目(2021XK046) （2021XK046）
