上海针灸杂志2025，Vol.44Issue(3)：355-365,11.DOI:10.13460/j.issn.1005-0957.2025.03.0355
针药并用对子宫内膜异位症疼痛大鼠CLEC5A、NLRP3和IL-1β表达的影响
Effects of the combination of acupuncture and medicines on the expression of CLEC5A,NLRP3 and IL-1β in rats with endometriosis pain
摘要
Abstract
Objective To observe the effects of the combination of acupuncture and medicines on the expression of CLEC5A,NLRP3 and IL-1β in rats with endometriosis pain,and to explore the mechanism of acupuncture and medicines in relieving endometriosis pain.Method Eight rats were randomly selected from 48 rats for sham operation as the sham operation group,and the remaining 40 rats were modeled for endometriosis.After the model was established,according to the total volume of the ectopic cysts,they were randomly divided into the model group,the ibuprofen group,the acupuncture-moxibustion group,the traditional Chinese medicines group,and the acupuncture plus medicines group,with 8 rats in each group.The sham-operated group and model group were fixed and given an equal amount of 0.9%saline by gavage,the ibuprofen group was fixed and given ibuprofen+0.9%saline by gavage,the acupuncture-moxibustion group was given fixed acupuncture-moxibustion intervention,the traditional Chinese medicines group was fixed and given Jiawei Mojie tablets+0.9%saline by gavage,and the acupuncture plus medicines group was given fixed acupuncture-moxibustion and Jiawei Mojie tablets+0.9%saline by gavage.The volume of ectopic cysts in rats with endometriosis was measured and calculated after modeling and intervention,and the pain thresholds of rats in the six groups were measured.The endometrial tissues of the six groups were examined for C-type lectin domain family 5 member A(CLEC5A),NOD-like receptor family pyrin domain containing protein 3(NLRP3),interleukin 1β(IL-1β)protein and mRNA expression levels.Result The values of volume reduction of ectopic cysts and increase in pain threshold after intervention in the acupuncture-moxibustion and acupuncture plus medicines groups were better than those in the model group(P＜0.05).The protein expression levels of CLEC5A,NLRP3,and IL-1β were higher than those of normal endometrial tissues in both in situ and ectopic endometrial tissues of the model group(P＜0.05).The protein expression levels of CLEC5A and NLRP3 in the in situ and ectopic endometrial tissues and IL-1β in the ectopic endometrial tissues of the acupuncture-moxibustion and acupuncture plus medicines groups were lower than those of the model group(P＜0.05),and the expression of IL-1β in the ectopic endometrium of the acupuncture-moxibustion group was significantly lower than that of the model group(P＜0.05).[The protein expression level of CLEC5A in the situ endothelial tissue of the acupuncture plus medicines group was lower than that of the ibuprofen group and the traditional Chinese medicines group(P＜0.05),and NLRP3 of the acupuncture plus medicines group was lower than that of the ibuprofen group(P＜0.05).]The protein expression level of CLEC5A in the ectopic endothelial tissue of the acupuncture plus medicines group was lower than that of the acupuncture-moxibustion group,the ibuprofen group and the traditional Chinese medicines group(P＜0.05),NLRP3 of the acupuncture plus medicines group was lower than that of the ibuprofen group and the traditional Chinese medicines group,and IL-1β of the acupuncture plus medicines group was lower than that of the ibuprofen group(P＜0.05).The levels of CLEC5A mRNA in the in situ and ectopic endometrium of the model group were higher than those in the endometrium of the sham operation group(P＜0.05),and the levels of IL-1β mRNA in the in situ endometrium of the model group were significantly higher than those in the endometrium of the sham operation group(P＜0.05).The level of CLEC5A mRNA in ectopic endometrium of ibuprofen group,acupuncture-moxibustion group,traditional Chinese medicines group and acupuncture plus medicines group was lower than that in the model group(P＜0.05);the level of NLRP3 and IL-1β mRNA in ectopic endometrium of traditional Chinese medicines group was lower than that in the model group(P＜0.05).Conclusion The combined use of acupuncture and medicines may reduce the volume of ectopic cysts and increase the pain threshold by down-regulating the expression of CLEC5A mRNA and protein and regulating the expression levels of NLRP3 and IL-1β protein in the tissues of the in situ and ectopic endometrium,thus alleviating the pain of endometriosis in rats.关键词
针药并用/子宫内膜异位症/疼痛/C型凝集素结构域家族5成员A/NLR家族Pyrin域蛋白3/白细胞介素-1β/⼤⿏Key words
Acupuncture medication combined/Endometriosis/Pain/C-type lectin structural domain family 5 member A/NOD-like receptor family pyrin domain containing protein 3/Interleukin-1β/Rats分类
医药卫生引用本文复制引用
胡佳玮,吴楚婷,徐丹丹,高源,李云翼,李铭旸,李语然,张春雁..针药并用对子宫内膜异位症疼痛大鼠CLEC5A、NLRP3和IL-1β表达的影响[J].上海针灸杂志,2025,44(3):355-365,11.基金项目
上海市"科技创新行动计划"启明星培育-扬帆专项(22YF1444200) （22YF1444200）
国家自然科学基金青年项目(82405565) （82405565）
上海市东方英才计划青年项目 （）
上海市卫生健康委员会卫生行业临床研究专项(202240054) （202240054）
金华市科学技术局重点项目(2024-3-140) （2024-3-140）
上海市"科技创新行动计划"科技成果转化服务体系建设项目(23ZC2520700) （23ZC2520700）
上海中医药大学"杏林学者" （）
上海申康医院发展中心促进市级医院临床技能与临床创新能力三年行动计划项目(SHDC2020CR3093B) （SHDC2020CR3093B）
