世界科学技术-中医药现代化2025，Vol.27Issue(2)：355-363,9.DOI:10.11842/wst.20241111001
"通督启神"针法调控细胞外基质改善SAMP8小鼠脑血流量提升学习记忆能力的机制研究
Study on the Mechanism of Regulating Extracellular Matrix by Tongdu Qishen Acupuncture to Improve Cerebral Blood Flow and Enhance Learning and Memory Ability in SAMP8 Mice
摘要
Abstract
Objective This study aims to investigate the effects of acupuncture on the spatial learning and memory capacities,the morphological characteristics of hippocampal neurons,the components of extracellular matrix,as well as the structure of the blood-brain barrier in SAMP8 mice.Moreover,it endeavors to explore the underlying mechanism through which the"Tongdu Qishen"(activating governor vessel and awakening spirit)acupuncture method ameliorates the cognitive function and modulates cerebral blood flow in SAMP8 mice.Methods Twenty 6-month-old male SAMP8 mice were randomly allocated into a model group and an electroacupuncture group,with ten mice in each group.Additionally,ten homologous 6-month-old male SAMR1 mice were designated as the normal control group.In the electroacupuncture group,the acupoint"Renzhong"(GV 26)was punctured using a quick insertion technique,while"Baihui"(GV 20)and"Yintang"(EX-HN 3)were punctured via a transverse insertion method.Subsequently,the needle handles were connected to an electroacupuncture apparatus,which was set to operate with a dense-sparse wave at a frequency of 100 Hz/2 Hz and an electric current of 2 mA.Each session of acupuncture lasted for 20 minutes and was conducted once a day for a consecutive period of 28 days.The normal group,the model group,and the electroacupuncture group all underwent identical fixation and restraint procedures to ensure consistent experimental conditions.The Morris water maze was employed to evaluate the spatial learning and memory abilities of the mice.The Nissl staining technique was utilized to assess the alterations in hippocampal neurons of SAMP8 mice.The laser speckle imaging system was adopted to measure the cerebral blood flow of the mice in each group.Transmission electron microscopy was applied to observe the ultrastructure of the blood-brain barrier within the frontal cortex tissue of the SAMP8 mice's brains.The Western blotting method was used to detect the expressions of Collagen-Ⅳ and Fibronectin in the brain tissues of SAMP8 mice.Results In comparison with the model group,the"Tongdu Qishen"acupuncture method demonstrated a significant efficacy in improving the spatial learning and memory abilities of SAMP8 mice(P<0.05).It also effectively alleviated the degeneration of hippocampal neurons,remarkably increased the cerebral blood flow perfusion volume of SAMP8 mice(P<0.05),ameliorated the damage to the blood-brain barrier structure,significantly upregulated the content of Collagen-Ⅳ(P<0.05),and significantly downregulated the content of Fibronectin(P<0.0001).Conclusion The"Tongdu Qishen"acupuncture method is capable of favorably regulating the extracellular matrix of SAMP8 mice,thereby improving cerebral blood flow and ultimately enhancing their learning and memory abilities.关键词
"通督启神"针法/针刺/阿尔茨海默病/快速老化小鼠/细胞外基质Key words
"Tongdu Qishen"acupuncture method/Acupuncture/Alzheimer's disease/Senescence Accelerated Mouse/Extracellular matrix分类
医药卫生引用本文复制引用
吴国庆,汪子栋,杨佳一,陶宜琳,姜婧,高效铭,李志刚.."通督启神"针法调控细胞外基质改善SAMP8小鼠脑血流量提升学习记忆能力的机制研究[J].世界科学技术-中医药现代化,2025,27(2):355-363,9.基金项目
国家自然科学基金面上项目(82274654):中性粒细胞黏附与内皮修复途径探讨"通督神"针法干预AD的血脑屏障保护机制研究,负责人:李志刚 （82274654）
北京中医药大学2022年度揭榜挂帅项目(2022-JYB-JBZR-024):穴位敏化的物质基础与成像研究,负责人:李志刚 （2022-JYB-JBZR-024）
国家自然科学基金面上项目(82174515):从"肠道菌群-神经炎症"探讨"通腑升清"针法保护AD神经元的作用机制,负责人:姜婧. （82174515）
