饲料研究2025，Vol.48Issue(2)：92-98,7.DOI:10.13557/j.cnki.issn1002-2813.2025.02.017
槟榔提取物及其生物碱脲酶抑制活性评价
Evaluation of Areca catechu extract and its alkaloids for urease inhibitory activity
摘要
Abstract
The experiment aims to investigate the inhibitory effects of Areca catechu extract(ANE)and its four main alkaloids(arecoline,arecaidine,guvacine,and guvacoline)on jack bean urease(JBU),using acetoxyhydroxamic acid(AHA)as a control,and to further explore the sites of their inhibitory action.The results showed that guvacoline(Guv)exhibited the most significant JBU inhibition effect with an IC50 value of 31.06 mg/L.Urease kinetics experiments showed that both ANE and Guv are hybrid inhibitors of JBU.The inhibitory effects of ANE and Guv on JBU were significantly reduced in the presence of thiol reagents(P＜0.05),and the inhibitory effects of Guv on JBU were significantly elevated in the presence of Ni2+competitive inhibitors(P＜0.05).Molecular docking results showed that Guv formed interactions with amino acid residues Asp 494,Ala 440,His 519,His 593,Arg 609,Gly 550,His 492,Ala 636,Asp 633,and His 409.The study indicates that Guv can effectively reduce the catabolism of urea by JBU,and Areca catechu possesses the potential for novel urease inhibitor functional product development.关键词
槟榔/洋刀豆脲酶/生物碱/脲酶抑制剂/分子对接Key words
Areca catechu/jack bean urease/alkaloids/urease inhibitors/molecular docking分类
畜牧业引用本文复制引用
颜倩,刘荣荣,束道燕,曾敏,曾建国..槟榔提取物及其生物碱脲酶抑制活性评价[J].饲料研究,2025,48(2):92-98,7.基金项目
国家重点研发计划(项目编号:2022YFD1801101) （项目编号:2022YFD1801101）
现代农业产业技术体系建设专项资金(项目编号:CARS-21) （项目编号:CARS-21）
