饲料研究2025，Vol.48Issue(2)：116-120,5.DOI:10.13557/j.cnki.issn1002-2813.2025.02.021
高胰岛素-正血糖钳夹技术测定围产期母羊胰岛素敏感性的研究
Study on insulin sensitivity of perinatal ewes measured by hyperinsulinemic-euglycemic clamp technique
摘要
Abstract
The study aimed to establish a method for detecting insulin(INS)sensitivity in periparturient ewes and to investigate the impact of ketosis on the energy balance of periparturient ewes.Experiment 1 selected three periparturient cashmere goats and subjected them to hyperinsulinemic-euglycemic clamp treatment.The plasma INS and glucose(GLU)levels of the cashmere goats were measured before and after infusion.Experiment 2 chose cashmere goats in the same periparturient period(seven days postpartum)as those in Experiment 1 from the same farm as the subjects.Six goats with ketosis[plasma β-hydroxybutyrate(BHBA)level≥1.6 mmol/L]and six goats without ketosis(plasma BHBA level＜0.8 mmol/L)were selected.Blood samples were collected from the jugular vein to detect the plasma levels of INS,GLU,BHBA,and non-esterified fatty acids(NEFA).The results showed that compared with the pre-treatment period,the plasma GLU level of the cashmere goats during the treatment period did not change significantly(P＞0.05),while the plasma INS level was extremely increased(P＜0.01).Throughout the experiment,the plasma INS level during the treatment period were higher than those before treatment.The ketotic goats had significantly higher plasma GLU,BHBA,and NEFA levels than the non-ketotic goats(P＜0.05),while their plasma INS level was significantly lower than those of the non-ketotic goats(P＜0.05).The study indicates that the hyperinsulinemic-euglycemic clamp technique is successfully used to detect INS sensitivity in periparturient ewes.关键词
围产期/母羊/胰岛素敏感性/高胰岛素-正血糖钳夹Key words
periparturient period/ewe/insulin sensitivity/hyperinsulinemic-euglycemic clamp分类
畜牧业引用本文复制引用
金鹿,桑丹,杨鼎,李文婷,付乐,宝华,胡晓晓,孙海洲..高胰岛素-正血糖钳夹技术测定围产期母羊胰岛素敏感性的研究[J].饲料研究,2025,48(2):116-120,5.基金项目
国家重点研发计划项目(项目编号:2022YFD1301102) （项目编号:2022YFD1301102）
国家绒毛用羊产业技术体系(项目编号:CARS-39-11) （项目编号:CARS-39-11）
国家自然科学基金(项目编号:32160802) （项目编号:32160802）
内蒙古自治区科技重大专项项目(项目编号:2021ZD0012) （项目编号:2021ZD0012）
内蒙古自治区草食动物营养科学重点实验室(项目编号:2022QNJJM03) （项目编号:2022QNJJM03）
农业农村部草食家畜健康养殖与畜产品品质调控重点实验室(部省共建)(项目编号:2023CXJJM08) （部省共建）
