饲料研究2025，Vol.48Issue(2)：134-138,5.DOI:10.13557/j.cnki.issn1002-2813.2025.02.024
类肠膜魏斯氏菌MbWp142冻干菌粉工艺优化及储藏稳定性研究
Study on process optimization and storage stability of lyophilized powder of Weissella paramesenteroides MbWp142
摘要
Abstract
The aim of the experiment was to optimize the process of freeze-dried bacteria powder of Weissella paramesenteroides MbWp142 and study its storage stability.The optimal centrifugation conditions for MbWp142 were first determined,followed by optimization of the lyophilized protective agent formula through single-factor and orthogonal experiments to enhance the survival rate of the lyophilized powder.The storage stability of the powder was also investigated.The results showed that the optimal centrifugation conditions were 8 000 r/min for 30 min.Under the conditions of 6%glucose,4%bacteriological peptone and 3%defatted milk,the survival rate of the lyophilized powder was 96.67%,which was 79.12%higher than that of the non-lyophilized powder,and the powder could be stored at-20℃for more than 28 days.The study indicates that the optimized process significantly enhanced the vitality of MbWp142 lyophilized powder,providing a reference for the preparation of probiotic feed additives.关键词
类肠膜魏斯氏菌/冻干保护剂/菌粉制备Key words
Weissella paramesenteroides/lyophilised protective agent/bacterial powder preparation分类
畜牧业引用本文复制引用
黄芳,吴佳敏,陈锶,洪真真,范铮,黄娟,黄燕燕..类肠膜魏斯氏菌MbWp142冻干菌粉工艺优化及储藏稳定性研究[J].饲料研究,2025,48(2):134-138,5.基金项目
国家自然科学基金(项目编号:32302242) （项目编号:32302242）
广东省科技创新战略专项资金(项目编号:2022B1212010015) （项目编号:2022B1212010015）
广东省基础与应用基础研究基金(项目编号:2022A1515110426) （项目编号:2022A1515110426）
广东省重点研发项目(项目编号:2023B0202040002) （项目编号:2023B0202040002）
