生物技术通报2025，Vol.41Issue(3)：14-24,11.DOI:10.13560/j.cnki.biotech.bull.1985.2024-1038
CO2还原用甲酸脱氢酶分子改造的研究进展
Advances on Molecular Modifications of Formate Dehydrogenase for CO2 Reduction
摘要
Abstract
With the urgent global demand for sustainable energy transformation and greenhouse gas emission reduction,the efficient green conversion of CO2 has become a research hotspot in various fields such as energy,environmental science,and chemical engineering.Especially in the context of combating climate change,CO2 capture and utilization is seen as one of the key strategies to achieve carbon neutrality.Formate dehydrogenase(FDH)as an important biocatalyst for reducing CO2 to formate,has shown significant potential in green chemistry and bioenergy conversion.However,it still has certain limitations regarding catalytic activity,thermal stability,and coenzyme specificity.In recent years,with the continuous development of protein engineering technology and molecular biology,researchers have proposed a variety of strategies to modify the performance of FDH,significantly enhancing its application prospects.For example,enzyme engineering means such as directed mutation and structure optimization can improve the substrate affinity of the enzyme,enhance the rigidity and conformational stability of the enzyme,and change the coenzyme binding site to broaden its application prospects in the process of green transformation.In this paper,the research progress of FDH catalytic CO2 reduction in recent years will be comprehensively summarized,focusing on the improvement measures of FDH in terms of catalytic efficiency,thermal stability,coenzyme specificity,etc.,the specific strategies adopted in the molecular transformation process will be discussed,and the rules of these strategies will be summarized,in order to provide new ideas and methods for the molecular transformation of FDH in the future and promote the development of its application in CO2 reduction reaction.In addition,with the development of artificial intelligence,machine learning,gene editing and other technologies,the molecular modification of FDH will be more efficient and precise in the future,and these emerging technologies are expected to screen out FDH mutants with excellent performance in a short time,providing a feasible green solution in solving the global climate change and energy crisis.关键词
CO2还原/甲酸脱氢酶/分子改造/催化效率/热稳定性/辅因子特异性/甲酸盐/NAD+/NADHKey words
CO₂ reduction/formate dehydrogenase/molecular modification/catalytic efficiency/thermal stability/cofactor specificity/formate/NAD⁺/NADH引用本文复制引用
陆峰,黄玉红,林燕娜,马富强..CO2还原用甲酸脱氢酶分子改造的研究进展[J].生物技术通报,2025,41(3):14-24,11.基金项目
国家重点研发计划(2021YFC2104200),江苏省自然科学基金青年基金项目(BK20240408),中国博士后科学基金面上资助项目(2024M762324),江苏省卓越博士后计划资助项目(2024ZB492),中国科学院青年创新促进会(2022327),山东省实验室项目(SYS202209) （2021YFC2104200）
