生物技术通报2025，Vol.41Issue(3)：35-43,9.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0667
γ射线诱变创制水稻突变体及其分子鉴定
Creation of Rice Mutant by Gamma-ray and Its Molecular Identification
摘要
Abstract
[Objective]Gamma-ray mutagenesis has been widely used in rice breeding because of its high efficiency and simple method.OsGRF4 is an important and rare pleiotropic gene in rice,which can significantly improve nitrogen use efficiency,yield and cold tolerance,while reduce seed shattering,this work aims to obtain different types of mutant resources and mine the regulatory genes of OsGRF4.[Method]The rice material NIL-GRF4 was mutated by γ rays in this study,the authenticity and genetic background of the mutants were identified by functional marker and SSR markers.[Result]By traits investigating,15 mutants of grain shape,6 mutants of zebra leaf,4 mutants of tiller and 3 mutants of sterile were obtained.Among them,an important grain type mutant was obtained.Its grain length reached 14.10 mm,and its grain length and 1 000-grain weight increased by 1.50 mm and 8.85 g respectively compared with the wild type,with an increase of 11.90%and 23.95%respectively.The authenticity and genetic background of the mutants were identified by using the functional markers of OsGRF4 and 48 pairs of SSR markers.The results showed that the mutants were all derived from wild-type NIL-GRF4,and the genetic background of the mutants was very similar to that of wild-type NIL-GRF4.[Conclusion]The F2 populations have been constructed by crossbreeding the mutants of grain shape,zebra leaf and sterile with the wild type,providing important gene resources for rice breeding.关键词
γ射线/OsGRF4/诱变育种/BSA-Seq/图位克隆/水稻Key words
gamma-ray/OsGRF4/mutation breeding/BSA-Seq/map-based cloning/rice引用本文复制引用
李欣芃,张武汉,张莉,舒服,何强,郭杨,邓华凤,王悦,孙平勇..γ射线诱变创制水稻突变体及其分子鉴定[J].生物技术通报,2025,41(3):35-43,9.基金项目
湖南省科技创新计划(2023NK2008),国家自然科学基金项目(32172000),湖南省农业科技创新资金(2023CX10),杂交水稻全国重点实验室基金(2023MS01) （2023NK2008）
