生物技术通报2025，Vol.41Issue(3)：51-61,11.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0898
紫外诱变选育巴弗洛霉素A1高产菌株及其培养基优化
The High-yield Bafilomycin A1 Strain Obtained from UV Mutagenesis and Its Medium Optimization
摘要
Abstract
[Objective]Bafilomycins are a class of macrolide compounds produced by the fermentation of Streptomyces griseobrunneus,which have antagonistic effect on a variety of plant fungi,and its representative compound is bafilomycin A1(Baf A1).In order to further enhance the yield of Baf A1 in Streptomyces sp.FIM-B0711 and reduce the production cost,the high-yielding strain of Baf A1 was selected and its fermentation process was optimized.[Method]High-yield mutant strains were generated through ultraviolet mutagenesis,followed by optimization of the fermentation process using single factor experiment,the steepest ascent experiment,and response surface methodology.[Result]The Baf A1 yield of the UV-21 strain reached 397.99 mg/L,representing a 23.60%increase compared to the original strain.Single factor experiment identified that maltodextrin,soybean peptone,and calcium carbonate were as the optimal carbon source,nitrogen source,and inorganic salt for strain UV-21,respectively.The optimal culture conditions were determined to be pH 7.0,an inoculum concentration of 3%(volume fraction),a liquid volume of 30 mL/250 mL,and a fermentation time of 96 h.The steepest ascent experiment revealed that the highest relative titer of 147.18%was achieved with a carbon source concentration of 45 g/L,nitrogen source concentration of 15 g/L,and inorganic salt concentration of 0.5 g/L.The response surface optimization demonstrated that the optimal fermentation process for the strain involved maltodextrin at 45.98 g/L,soybean peptone at 14.96 g/L,calcium carbonate at 0.48 g/L,valine at 3 g/L,an initial pH of 7.0,an inoculum concentration of 3%(volume fraction),a liquid volume of 30 mL/250 mL,and a fermentation time of 96 h.Under these optimized conditions,the fermentation titer reached 627.58 mg/L,a 57.69%increase compared to the initial process.[Conclusion]A high-yield Baf A1 strain with 627.58 mg/L is obtained based on ultraviolet mutagenesis and medium response surface optimization experiment,which increases the yield by 57.69%compared to the original strain FIM-B0711.关键词
巴弗洛霉素/链霉菌/响应面试验/发酵优化/紫外诱变/培养条件/工艺参数Key words
bafilomycins/Streptomyces/response surface test/fermentation optimization/ultraviolet mutagenesis/cultivation condition/process parameters引用本文复制引用
陈海敏,孙菲,袁源,吴佳雯,江红,周剑..紫外诱变选育巴弗洛霉素A1高产菌株及其培养基优化[J].生物技术通报,2025,41(3):51-61,11.基金项目
福建省属公益类科研院所基本科研专项(2024R1005007,2023R1005004) （2024R1005007,2023R1005004）
