生物技术通报2025，Vol.41Issue(3)：83-89,7.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0813
5′UTR区的编辑降低大麦GBSSI基因表达
Reducing the Expression of GBSSI Gene in Barley via the Editing in the 5′UTR Region
摘要
Abstract
[Objective]Barley is the fourth largest grain crop.The total starch content and the ratio of amylose and amylopectin in the grain are the key factors to determine the quality and characteristics of barley.The fine control of amylose content is of great significance for the improvement of barley flour quality.[Method]In this study,barley Cas9 overexpression lines were used as receptors,and the 5′non-coding region(5′ UTR)of GBSSI gene was targeted by BSMV-SG-mediated gene editing system to achieve the fine regulation of GBSSI gene expression.[Result]Our results indicated that somatic editing occurred in the leaves of the fifth tiller from 14 M0 generation 5′UTR plants,with an editing efficiency ranging from 1.22%to 84.49%.Seeds from the fifth tiller exhibiting peak editing efficiency were harvested,leading to identification of six edited lines among twenty-three M1 generation plants,resulting in an edited plant proportion of 26.08%.RT-qPCR analysis revealed a reduction in GBSSI gene expression by 40%-82%compared to controls.[Conclusion]These findings demonstrate that GBSSI gene expression can be effectively regulated through modification of its 5′UTR region,providing novel insights into precise regulation mechanisms for amylose synthesis.关键词
基因编辑/BSMV-sg系统/5′UTR/GBSSI基因/表达调控/大麦Key words
gene editing/BSMV-sg system/5′UTR/GBSSI gene/expression regulation/barley引用本文复制引用
薛瑞莹,刘永菊,姜燕燕,彭欣雅,曹东,李云,刘宝龙,包雪梅..5′UTR区的编辑降低大麦GBSSI基因表达[J].生物技术通报,2025,41(3):83-89,7.基金项目
青海省创新平台建设专项青藏高原种质资源研究与利用实验室(2024) （2024）
