生物技术通报2025，Vol.41Issue(3)：90-97,8.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0435
大豆GmPM31基因启动子响应高温高湿胁迫的功能分析
Functional Analysis of Soybean GmPM31 Gene Promoter Involvement in Response to High Temperature and Humidity Stress
摘要
Abstract
[Objective]To study the function of GmPM31 promoter on seed vigor formation in response to high temperature and high humidity stress,and to lay a foundation for comprehensively unravelling the function of soybean small heat shock proteins involved in the response to high temperature and high humidity stress.[Method]Homozygous T3 transgenic Arabidopsis thaliana with GmPM31 promoter was treated with high temperature and high humidity stress(40℃/100%relative humidity),and wild-type A.thaliana was used as control to detect its tolerance and stomatal opening.Analysis of GUS gene expression driven by the soybean GmPM31 promoter by GUS histochemical staining and quantitative real-time PCR.The seed vigor was detected by 2,3,5-triphenyltetrazolium chloride(TTC)staining.[Result]Compared with the control group,the tolerance of GmPM31-transgenic promotor A.thaliana to high temperature and high humidity increased.The activity of GUS increased,and they were expressed in the leaves,roots and flowers,and germination rate and vigor increased.[Conclusion]GmPM31 promoter improve the resistance of seeds to high temperature and high humidity stress and the ability to resist deterioration.关键词
大豆/启动子/高温高湿/种子活力Key words
soybean/promoter/high temperature and high humidity/seed vigor引用本文复制引用
林紫依,吴一舟,叶芳贤,朱淑颖,刘燕敏,刘骕骦..大豆GmPM31基因启动子响应高温高湿胁迫的功能分析[J].生物技术通报,2025,41(3):90-97,8.基金项目
国家级大学生创新创业训练计划资助项目(202313287004),湖州学院大学生创新创业训练科研项目(2023CXCY119,2023CXCY128) （202313287004）
