生物技术通报2025，Vol.41Issue(3)：98-103,6.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0721
花生蛋白磷酸酶AhPDCP37的抗旱性功能研究
Mechanism of Tolerance of Protein Phosphatase AhPDCP37 in Peanut to Drought
摘要
Abstract
[Objective]To investigate the mechanism of tolerance of the peanut protein phosphatase AhPDCP37 to drought under dehydration stress,and to provide a research basis for biotechnological means of modifying new plant varieties.[Method]The phenotype and leaf stomatal opening of transgenic Arabidopsis plants overexpressing AhPDCP37 were observed under drought conditions,and physiological and biochemical characteristics as well as changes in the expression of genes related to the response to drought stress and those related to the ABA signaling pathway were measured.[Result]The malondialdehyde content in Arabidopsis overexpressing AhPDCP37 was less up-regulated under drought stress compared with the wild type,whereas superoxide dismutase and peroxidase activities showed a significant up-regulation of expression under drought treatment.Meanwhile,leaf stomatal openings showed a decreasing trend in all groups under drought stress,but the decrease in the overexpression of AhPDCP37 treatment group was significantly lower than that in the wild-type treatment group.This indicates that AhPDCP37 enhanced the survival ability of plants under drought conditions by decreasing stomatal opening and increasing the content of cellular antioxidant enzymes.Under drought conditions,the expressions of positively regulated genes(WDR55,APA1)and genes related to ABA signaling pathway(NCED3,ABF3,and RD29A)were significantly up-regulated in the Arabidopsis treatment group overexpressing AhPDCP37 compared with the wild-type treatment group,and the expressions of negatively regulated genes of drought stress response WRKY70 significantly decreased compared with the wild-type treatment group.[Conclusion]AhPDCP37 improves tolerance in plants to drought under drought stress conditions mainly through its involvement in the regulation of stomatal movement and antioxidant enzyme activities.关键词
AhPDCP37/干旱胁迫/ABA/抗氧化酶/气孔开度Key words
AhPDCP37/drought stress/ABA/antioxidant enzyme/stomatal opening引用本文复制引用
钱祺,王增辉,孙荣华,罗英智,苏良辰..花生蛋白磷酸酶AhPDCP37的抗旱性功能研究[J].生物技术通报,2025,41(3):98-103,6.基金项目
国家自然科学基金地区项目(31860310),贵州省科技厅自然科学基金项目(黔科合基础-ZK一般518) （31860310）
