生物技术通报2025，Vol.41Issue(3)：104-111,8.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0839
陆地棉GhNFD4在棉花干旱响应中的功能
Functional Studies of the Gossypium hirsutum GhNFD4 Gene in Response to Drought in Cotton
摘要
Abstract
[Objective]To analyze the biological function of GhNFD4 in response to drought stress in terrestrial cotton,and to provide genetic resources and theoretical basis for molecular breeding of terrestrial cotton for stress tolerance.[Method]Real-time fluorescence quantitative PCR(RT-qPCR)was used to detect the expression pattern of GhNFD4 in cotton seedlings under PEG-mimicked natural drought stress;tobacco crisped virus(TRV)-induced gene silencing(VIGS)was utilized to explore the regulatory role of GhNFD4 in drought stress,and the interfering sequences of GhNFD4 were cloned and the gene silencing vectors were constructed.The GhNFD4 silencing plants were further obtained by infiltrating cotton seedlings through transient transfection technology,observing the phenotypic differences between silencing plants and control plants at 15 d of natural water loss,detecting the accumulation of ROS products by using NBT and DAB staining,and detecting the water loss rate and drought-related physiological indexes of isolated leaves.[Result]The expression of GhNFD4 was induced by natural drought stress simulated by PEG and peaked at the 12th h of stress.After 15 d of natural drought,the leaves of GhNFD4-silenced plants showed more severe wilting and darker coloring after NBT and DAB staining compared with the negative control plants,and the water loss rate of the isolated leaves and the relative electrical conductivity(REL),malondialdehyde(MDA),and hydrogen peroxide(H2O2)contents of the leaves after drought stress were significantly higher compared with the control,and the total antioxidant enzyme(T-AOC)activity was significantly lower compared with the control activity was significantly decreased compared with the control group.[Conclusion]GhNFD4 is induced by drought stress and actively participates in the positive regulation of drought stress in cotton by regulating the activity of antioxidant enzymes under drought conditions,which improves the resistance of cotton to drought stress.关键词
棉花/GhNFD4/干旱/基因表达/VIGSKey words
Gossypium hirsutum/GhNFD4/drought/gene expression/VIGS引用本文复制引用
马利花,侯梦娟,朱新霞..陆地棉GhNFD4在棉花干旱响应中的功能[J].生物技术通报,2025,41(3):104-111,8.基金项目
国家自然科学基金项目(32360457) （32360457）
