生物技术通报2025，Vol.41Issue(3)：161-170,10.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0722
白菜型油菜CCDs家族全基因组鉴定及表达分析
Genome-wide Identification and Expression Analysis of CCDs Family in Brassia rapa L.
摘要
Abstract
[Objective]The members of the carotenoid cleavage dioxygenase gene family in Brassia rapa L.were identified to gain insight into the function and tissue expression characteristics of the BrCCD family genes.[Method]Bioinformatics methods were used to identify the CCDs gene family in B.rapa L.,construct a phylogenetic tree,and analyze the chromosome distribution,gene structure,conserved motif,intraspecific collinearity and promoter cis elements.Combined with transcriptome data and RT-qPCR technology,the expression of CCDs genes in different tissues was analyzed.[Result]Total 16 BrCCD genes were identified in B.rapa L.,which were divided into six subgroups and distributed unevenly on eight chromosomes.Colinearity analysis showed that there was a collinearity between seven pairs of CCDs genes.Cis element analysis showed that CCDs family members may respond to various regulatory processes such as growth and development,hormone regulation,and abiotic stress.Transcriptome and quantitative PCR data analysis showed that the expression of BrCCD was tissue-specific,and the expression of BrCCD-L gene was higher in flowers,leaves and seeds,BrCCD1b was higher in flowers,and the rest was higher in seeds and leaves.[Result]Total 16 CCDs genes were identified in the genome of B.rapa L.,which showed different expression patterns in different tissues.The BrCCD-L gene was highly similar to the CCD2 gene with saffron genus,and was significantly expressed in different tissues.关键词
白菜型油菜/生物信息学分析/CCDs基因家族/表达分析/组织特异性Key words
Brassia rapa L./bioinformatics analysis/CCDs gene family/expression analysis/tissue specificity引用本文复制引用
王琛,刘国梅,陈畅,张晋龙,姚琳,孙璇,杜春芳..白菜型油菜CCDs家族全基因组鉴定及表达分析[J].生物技术通报,2025,41(3):161-170,10.基金项目
山西省科技重大专项计划(202201140601025-5),山西省基础研究计划项目(202203021211276),山西农业大学生物育种工程项目(YZGC010) （202201140601025-5）
