生物技术通报2025，Vol.41Issue(3)：190-201,12.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0820
'红阳'猕猴桃TCP基因家族鉴定及其在果实中的表达分析
Identification of the TCP Gene Family in Actinidia chinensis var.Hongyang and Their Expression Analysis in Fruit
摘要
Abstract
[Objective]The members of TEOSINTE-BRANCHED1/CYCLOIDEA/PCF in kiwifruit'Hongyang'(Actinidia chinensis var.Hongyang)(AcTCPs)gene family were identified based on whole-genome.Taxonomic and evolutionary relationships of the members were analyzed,and the expression profiles of the AcTCP genes in tissue-specific expression,fruit development process,and hormones treatments were explored.These findings provide a basis for further exploring the function of the AcTCP gene in kiwifruit.[Method]Bioinformatics methods were used to analyze the physicochemical properties,gene structure,conserved motifs,cis-acting elements and inter-species covariance of TCP transcription factors in kiwifruit'Hongyang'were analyzed.RNA-seq and RT-qPCR were used to investigate the expression profiles of AcTCPs in different tissues,fruit development and in response to hormonal stimuli.[Result]A total of 40 AcTCP genes with complete TCP domains were identified on 19 chromosomes in kiwifruit'Hongyang'and named AcTCP1-AcTCP40.Using phylogenetic analyses,conserved motif,and gene structure analyses,the family members were divided into PCF(21),CIN(12)and CYC/TB1(7).Synteny analysis showed that genome-wide or segment-based replication played an important role in the expansion of the kiwifruit'Hongyang'TCP gene family.Analysis of tissue-specificity showed that the six candidate genes expressed significantly high in male flowers and fruits.The results of RT-qPCR experiments at different stages of fruit development found that AcTCP1 showed elevated expressions at later stages of fruit development,while AcTCP11,AcTCP35,AcTCP10,and AcTCP32 demonstrated high expression during the initial stages of fruit development,and the expression pattern was consistent with the transcriptomic results.In fruit,abscisic acid(ABA)and ethylene(ET)treatments to the fruits down-regulated six candidate AcTCPs,gibberellin(GA3)treatment up-regulated the expressions of AcTCP1,AcTCP35 and AcTCP32,and the forchlorfenuron(CPPU)treatment up-regulated the expressions of AcTCP1,AcTCP35 and AcTCP32 in initial stages,whereas GA3 and CPPU decreased the expressions of the others AcTCPs.[Conclusion]A total of 40 TCP members from kiwifruit'Hongyang'were systematically identified.The expression patterns of six candidate genes highly express in the fruit and male flowers.The expressions of AcTCP35,AcTCP32,AcTCP1,AcTCP10,AcTCP11 and AcTCP20 in the fruit are involved in kiwifruit development and are regulated by various exogenous hormones(ABA,ET,GA3,and CPPU).关键词
'红阳'猕猴桃/TCP转录因子/全基因组鉴定/生物信息学/组织差异/表达模式分析/激素处理Key words
Actinidia chinensis var.Hongyang/TCP transcription factors/genome-wide identification/bioinformatics/organizational differences/expression pattern analysis/hormone treatment引用本文复制引用
宋姝熠,蒋开秀,刘欢艳,黄亚成,刘林娅..'红阳'猕猴桃TCP基因家族鉴定及其在果实中的表达分析[J].生物技术通报,2025,41(3):190-201,12.基金项目
六盘水师范学院科研培育项目(LPSSY2023KJZDPY07),国家自然科学基金项目(32360740),贵州省教育厅自然科学研究项目(黔教技[2022]054号),六盘水市科技计划项目(52020-2022-PT-03,52020-2023-0-2-15),大学生创新训练计划项目(202310977011) （LPSSY2023KJZDPY07）
