山西医科大学学报2025，Vol.56Issue(1)：8-14,7.DOI:10.13753/j.issn.1007-6611.2025.01.002
MAPK4表达对黑色素瘤细胞增殖和凋亡的影响
Effect of MAPK4 expression on proliferation and apoptosis of melanoma cells
摘要
Abstract
Objective To explore the expression of mitogen-activated protein kinase 4(MAPK4)in melanoma and its effect and mo-lecular mechanisms on cell proliferation,cell cycle and apoptosis.Methods qRT-PCR and immunohistochemistry were employed to detect MAPK4 expression in adjacent non-tumor tissues and melanoma tissues.A2058 cells were divided into negative control group,siRNA-1 group and siRNA-2 group,and transfected with NC-siRNA,MAPK4 siRNA1 and MAPK4 siRNA2,respectively.The cell proliferation activity was assessed using MTT method at 12,36,60,84 h after transfection,and the cell proliferation was detected by cell cloning assay.Cell cycle and apoptosis were analyzed by flow cytometry.The expressions of MAPK4 and AKT proteins were de-tected by Western blotting.Results Both MAPK4 mRNA and protein expressions were significantly increased in melanoma tissues compared to adjacent non-tumor tissues(P<0.01).Compared with NC-siRNA group,the cell proliferation was significantly reduced in MAPK4 siRNA-1 group and MAPK4 siRNA-2 group(P<0.01),the percentage of G0/G1 phase cells was significantly increased,the percentage of S phase cells was decreased(P<0.01),the early and late apoptotic rates were increased(P<0.01),and MAPK4 and AKT protein expressions were downregulated(P<0.01).Conclusion MAPK4 expression is upregulated in melanoma tissues,and silencing MAPK4 may inhibit melanoma cell proliferation and cell cycle transition,and promote apoptosis through modulating AKT signaling pathway.关键词
MAPK4/黑色素瘤/细胞增殖/细胞周期/细胞凋亡/AKT信号通路Key words
MAPK4/melanoma/cell proliferation/cell cycle/cell apoptosis/AKT signaling pathway分类
临床医学引用本文复制引用
赵晟辰,邓朝伟,刘乐凡,郑琪,张珂浛,赵凌宇,吴锋..MAPK4表达对黑色素瘤细胞增殖和凋亡的影响[J].山西医科大学学报,2025,56(1):8-14,7.基金项目
陕西省重点研发计划项目(2023-YBSF-178) （2023-YBSF-178）
国家级大学生创新训练计划项目(202310698152) （202310698152）
