山西医科大学学报2025，Vol.56Issue(1)：28-35,8.DOI:10.13753/j.issn.1007-6611.2025.01.005
基于NLRP3/ASC/Caspase-1信号通路探讨肝复胶囊抗慢性肝损伤的作用机制
Effect and mechanism of Ganfu capsule against chronic liver injury based on NLRP3/ASC/Caspase-1 signaling pathway
摘要
Abstract
Objective To study the effect and mechanism of Ganfu capsule(GFC)against CCl4-induced chronic liver injury(CLI)in rats.Methods Sixty SD male rats were randomly divided into control group,model group,Colchicine group(2×10-4 g/kg)and Ganfu capsule low dose group(GFC-L,0.781 3 g/kg),medium dose group(GFC-M,1.562 5 g/kg),and high dose group(GFC-H,3.125 g/kg),with 10 rats in each group.The rats in control group were not treated,and the rats in the other groups were subcuta-neously injected with 40%CCl4 peanut oil mixture(3 mL/kg,except for the first dose of 5 mL/kg)on the dorsal surface twice a week for 12 weeks.At week 4,the rats in drug groups were gavaged with the corresponding drugs once a day,and the rats in control group and model group were gavaged with the same amount of saline.At the end of 12 weeks,the rats were weighed and then sacrificed to collect the blood from the abdominal aorta and the livers.HE staining and Masson staining were used to observe the pathological changes of liver tissues.Biochemical kits were used to detect serum levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST).ELISA was used to detect the expressions of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and IL-6.Western blot was used to detect the protein expression levels of nod-like receptor protein 3(NLRP3),apoptosis associated speck-like protein contain-ing a caspase activating recruitment domain(ASC),and cysteinyl aspartate specific proteinase-1(Caspase-1).Results Compared with model group,the body mass was significantly increased in GFC groups and Colchicine group,the inflammatory infiltration and collagen deposition in liver tissues were reduced,the serum levels of ALT,AST,TNF-α,IL-1β,and IL-6 were decreased(P<0.05),and the protein expressions of NLRP3,ASC,and Caspase-1 in liver tissues were downregulated(P<0.05),and the efficacy of high dose of GFC for improving CLI was better than that of Colchicine.Conclusion GFC may alleviate the CCl4-induced hepatic inflam-matory injury and exert hepatoprotective effects by inhibiting the expression of inflammatory factors through the downregulation of NLRP3/ASC/Caspase-1 signaling pathway.关键词
肝复胶囊/慢性肝损伤/NLRP3/ASC/Caspase-1/炎症Key words
Ganfu capsule/chronic liver injury/NLRP3/ASC/Caspase-1/inflammation分类
临床医学引用本文复制引用
李敏仪,黄以蓉,彭凯,彭继,谢锋,王祁,高璐,李敏..基于NLRP3/ASC/Caspase-1信号通路探讨肝复胶囊抗慢性肝损伤的作用机制[J].山西医科大学学报,2025,56(1):28-35,8.基金项目
秦创原中药创新研发项目(2022-QCYZH-050) （2022-QCYZH-050）
陕西中医药大学校级高水平中医药重点学科建设-临床中药学项目(2024XKZD12) （2024XKZD12）
