山西医科大学学报2025，Vol.56Issue(1)：43-48,6.DOI:10.13753/j.issn.1007-6611.2025.01.007
川崎病高表达SEMA7A促进单核和中性粒细胞黏附与迁移
High expression of SEMA7A in Kawasaki disease promotes adhesion and migration of monocytes and neutrophils
摘要
Abstract
Objective To investigate the role of Semaphorin 7A(SEMA7A)in the adhesion and the transendothelial migration of monocytes(THP-1 cell line)and neutrophils(HL-60 cell line)in Kawasaki disease(KD).Methods For in vivo experiment,four-week-old male C57BL/6 mice were randomly divided into control group and model group,with 6 mice in each group.The mice in model group were intraperitoneally injected with lactobacillus casei cell wall extract(LCWE)to establish a KD-like vasculitis mouse model,while the mice in control group were injected with phosphate-buffered saline(PBS).At day 14 after injection,the expression of SEMA7A in cardiac tissue was detected by real-time quantitative PCR(RT-PCR)and Western blot.For in vitro experiment,human coronary artery endothelial cells(HCAECs)were divided into healthy children's serum(HC)stimulation group and KD serum stimulation group.After 24 h culture,the expression level of SEMA7A was detected by RT-PCR and Western blot.Moreover,HCAECs stimulated by KD serum were further divided into si-NC group and si-SEMA7A group,and the SEMA7A expression was detected by RT-PCR.Furthermore,the HCAECs from four groups were respectively seeded into the upper chamber of Transwell device to form a monolayer and co-cultured with THP-1 or HL-60,then the adhesion and the transendothelial migration numbers of THP-1 and HL-60 were detected by fluorescence labeling and Transwell experiment.Results Compared with control group,the mRNA and protein expressions of SEMA7A in cardiac tissue of model group were increased(P<0.01).The mRNA and protein expressions of SEMA7A in KD serum stimulation group were both higher than those in HC serum stimulation group(P<0.01).Additionally,the mRNA expression of SEMA7A in si-SEMA7A group was lower than that in si-NC group(P<0.01).Moreover,the numbers of THP-1 and HL-60 adhering to HCAEC monolayer in KD serum stimulation group were higher than those in HC serum stimulation group(P<0.05),and the numbers in si-SEMA7A group were lower than those in si-NC group(P<0.05).Furthermore,the numbers of THP-1 and HL-60 migrating across the endothelial cells in KD serum stimulation group were higher than those in HC serum stimulation group(P<0.01),and the numbers in si-SEMA7A group were significantly decreased compared with si-NC group(P<0.05).Conclusion High expression of SEMA7A under KD conditions could promote the adhesion and the transendothelial cell migration of monocytes and neutrophils.关键词
信号素7A/川崎病/单核细胞/中性粒细胞/内皮细胞/细胞黏附/迁移Key words
Semaphorin 7A/Kawasaki disease/monocyte/neutrophil/endothelial cell/cell adhesion/migration分类
临床医学引用本文复制引用
黄君华,党心睿,方可,苏以萱,李一阳,张书婉..川崎病高表达SEMA7A促进单核和中性粒细胞黏附与迁移[J].山西医科大学学报,2025,56(1):43-48,6.基金项目
陕西省自然科学基础研究计划项目(2024JC-YBQN-0869,2023-JC-YB-718) （2024JC-YBQN-0869,2023-JC-YB-718）
西安市科技计划项目(24YXYJ0064) （24YXYJ0064）
西安市卫生健康委员会科研项目(2023qn12) （2023qn12）
陕西省大学生创新创业训练计划项目(S202311840077,S202311840070) （S202311840077,S202311840070）
西安医学院大学生创新创业训练计划项目(121523077,121523070) （121523077,121523070）
