山西医科大学学报2025，Vol.56Issue(1)：49-56,8.DOI:10.13753/j.issn.1007-6611.2025.01.008
基于MAPK/NF-κB信号通路探究石杉碱甲对去卵巢小鼠骨质疏松的作用
Effect and mechanism of huperzine A on osteoporosis in ovariectomized mice based on MAPK/NF-κB signaling pathway
摘要
Abstract
Objective To investigate the effect and mechanism of huperzine A on osteoporosis in ovariectomized mice.Methods Forty female C57BL/6 mice were randomly divided into sham group,OVX group,huperzine A low-dose group(SSJJ-L,0.05 mg/kg)and huperzine A high-dose group(SSJJ-H,0.2 mg/kg).The osteoporosis model was established by bilateral ovariectomy.After two months of modeling,the levels of tumor necrosis factor-α(TNF-α),interleukin 1β(IL-1β),IL-6,and IL-10 in serum were detected by ELISA.Alkaline phosphatase(ALP)and tartrate-resistant acid phosphatase(StrACP)levels in serum were detected by chemilumines-cence assay.Dynamic changes of bone trabecular area and bone formation in upper tibia were observed under the microscope.The biome-chanics of femur were measured by compression method and the expression level of key proteins in MAPK/NF-κB pathway was detected by Western blot.Results Compared with sham group,the serum levels of TNF-α,IL-1β,IL-6,IL-10,StrACP and ALP were sig-nificantly increased in OVX group(P<0.01),the percentage of trabecular bone area in upper tibia was significantly decreased(P<0.01),the double fluorescence distance was significantly increased(P<0.01),the maximum load and the fracture load of femur were significantly decreased(P<0.01),and the relative expression levels of p-p38 MAPK and p-NF-κB p65 were significantly increased(P<0.01).Compared with OVX group,the serum levels of TNF-α,IL-1β,IL-6,IL-10,StrACP and ALP were significantly decreased in huperzine A group(P<0.01),the percentage of bone trabecular area in upper tibia was significantly increased(P<0.01),the double fluorescence distance was significantly decreased(P<0.01),the maximum load and the fracture load of femur were significantly in-creased(P<0.01),and the relative expression levels of p-p38 MAPK and p-NF-κB p65 protein were significantly decreased(P<0.01).Compared with huperzine A low-dose group,the above indexes in huperzine A high-dose group were significantly improved(P<0.05).Conclusion Huperzine A may regulate MAPK/NF-κB signaling pathway to reduce the inflammation,inhibit the bone resorption and promote the bone formation,and ameliorate the osteoporosis.关键词
石杉碱甲/骨质疏松/小鼠/卵巢摘除/MAPK/NF-κB信号通路/炎症Key words
huperzine A/osteoporosis/mice/ovariectomy/MAPK/NF-κB signaling pathway/inflammation分类
临床医学引用本文复制引用
郑锦畅,谢斌,黄成硕,林思恩,梁振,魏波,胡资兵,林瀚,孙杰聪,郭伟雄,陈驹..基于MAPK/NF-κB信号通路探究石杉碱甲对去卵巢小鼠骨质疏松的作用[J].山西医科大学学报,2025,56(1):49-56,8.基金项目
广东省医学科学技术研究基金项目(B2024110,B2024006) （B2024110,B2024006）
湛江市科技发展专项资金竞争性分配项目(2022A01176) （2022A01176）
